Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging

Chia Yuan Chang, Cheng Han Lin, Chun Yu Lin, Yong Da Sie, Yvonne Yuling Hu, Sheng Feng Tsai, Shean Jen Chen

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

A developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3.0 μm to 1.5 μm for a 50 % improvement. By using the TFMPEM with HiLo technique as two structured illuminations at the same spatial frequency but different orientation, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression. Picture: TPEF images of the eosin-stained mouse cerebellar cortex by conventional TFMPEM (left), and the TFMPEM with HiLo technique as 1.09 μm−1 spatially modulated illumination at 90° (center) and 0° (right) orientations. (Figure presented.).

原文English
文章編號e201600287
期刊Journal of Biophotonics
11
發行號1
DOIs
出版狀態Published - 2018 一月

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 材料科學(全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 工程 (全部)
  • 物理與天文學 (全部)

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