Testosterone synthesized in cultured human SZ95 sebocytes derives mainly from dehydroepiandrosterone

Wen Chieh Chen, Shaw Jenq Tsai, Hamm Ming Sheu, Jih Chien Tsai, Christos C. Zouboulis

研究成果: Letter同行評審

34 引文 斯高帕斯(Scopus)

摘要

Human sebaceous gland possesses all the steroidogenic enzymes required for androgen synthesis. It remains unclear whether the testosterone produced in situ mainly derives from circulating dehydroepiandrosterone (DHEA) or from de novo synthesis utilizing serum cholesterol. Using testosterone radioimmunoassay, we found that testosterone was barely detectable in the supernatant of cultured human SZ95 sebocytes when cholesterol was added alone, indicating a low basal expression of steroidogenic acute regulatory protein (StAR) in SZ95 cells. Human chorionic gonadotropin and fibroblast growth factor-9 were as potent as forskolin in activating StAR to enhance testosterone production, while interleukin-1β, dexamethasone, insulin and insulin-like growth factor-1 showed no stimulatory effect. A two-fold increase of testosterone production was observed in supplementation of DHEA as compared to pregnenolone, progesterone or 17α-hydroxyprogesterone. Based on our findings, testosterone synthesized in cultured sebocytes derived mainly from DHEA and inhibition of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase may be a new target of androgen suppression for acne treatment.

原文English
頁(從 - 到)470-472
頁數3
期刊Experimental Dermatology
19
發行號5
DOIs
出版狀態Published - 2010 五月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學
  • 皮膚科

指紋

深入研究「Testosterone synthesized in cultured human SZ95 sebocytes derives mainly from dehydroepiandrosterone」主題。共同形成了獨特的指紋。

引用此