The Birt-Hogg-Dubé tumor suppressor folliculin negatively regulates ribosomal RNA synthesis

Kriti Gaur, Jinghong Li, Dakun Wang, Pranabananda Dutta, Shian Jang Yan, Amy Tsurumi, Hartmut Land, Guan Wu, Willis X. Li

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

Birt-Hogg-Dubé syndrome (BHD) is a human cancer disorder caused by mutations in the tumor suppressor gene Folliculin (FLCN) with unknown biological functions. Here, we show that the Drosophila homolog of FLCN, dFLCN (a.k.a. dBHD) localizes to the nucleolus and physically interacts with the 19S proteasomal ATPase, Rpt4, a nucleolar resident and known regulator of rRNA transcription. Downregulation of dFLCN resulted in an increase in nucleolar volume and upregulation of rRNA synthesis, whereas dFLCN overexpression reduced rRNA transcription and counteracted the effects of Rpt4 on rRNA production by preventing the association of Rpt4 with the rDNA locus. We further show that human FLCN exhibited evolutionarily conserved function and that Rpt4 knockdown inhibits the growth of FLCN-deficient human renal cancer cells in mouse xenografts. Our study suggests that FLCN functions as a tumor suppressor by negatively regulating rRNA synthesis.

原文English
文章編號dds428
頁(從 - 到)284-299
頁數16
期刊Human Molecular Genetics
22
發行號2
DOIs
出版狀態Published - 2013 1月

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學
  • 遺傳學(臨床)

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