The clinical implication of vitamin D nanomedicine for peritoneal dialysis-related peritoneal damage

Yi Che Lee, Chih Ting Huang, Fong Yu Cheng, Shih Yuan Hung, Tsun Mei Lin, Yen Chang Tsai, Chih I. Chen, Hao Kuang Wang, Chi Wei Lin, Hung Hsiang Liou, Min Yu Chang, Hsi Hao Wang, Yuan Yow Chiou

研究成果: Article

摘要

Purpose: Vitamin D is a novel potential therapeutic agent for peritoneal dialysis (PD)-related peritoneal fibrosis, but it can induce hypercalcemia and vascular calcification, which limits its applicability. In this study, we create nanotechnology-based drug delivery systems to investigate its therapeutics and side effects. Materials and methods: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino-(polyethylene glycol)2000] (DSPE-PEG) and L-α-phosphatidylcholine (PC), which packages with 1α,25(OH)2D3, were used to construct vitamin D nanoliposomes. To confirm the function and safety of vitamin D nanoliposomes, peritoneal mesothelial cells were treated with TGF-β1 and the reverse was attempted using vitamin D nanoliposomes. Antibodies (Ab) against the peritoneum-glycoprotein M6A (GPM6A) Ab were conjugated with vitamin D nanoliposomes. These particles were implanted into mice by intraperitoneal injection and the animals were monitored for the distribution and side effects induced by vitamin D. Results: Vitamin D nanoliposomes were taken up by the mesothelial cells over time without cell toxicity and it also provided the same therapeutic effect in vitro. In vivo study, fluorescent imaging showed vitamin D nanoliposomes allow specific peritoneum target effect and also ameliorate vitamin D side effect. Conclusion: Nanoliposomes vitamin D delivery systems for the prevention of PD-related peritoneal damage may be a potential clinical strategy in the future.

原文English
頁(從 - 到)9665-9675
頁數11
期刊International journal of nanomedicine
14
DOIs
出版狀態Published - 2019

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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    Lee, Y. C., Huang, C. T., Cheng, F. Y., Hung, S. Y., Lin, T. M., Tsai, Y. C., Chen, C. I., Wang, H. K., Lin, C. W., Liou, H. H., Chang, M. Y., Wang, H. H., & Chiou, Y. Y. (2019). The clinical implication of vitamin D nanomedicine for peritoneal dialysis-related peritoneal damage. International journal of nanomedicine, 14, 9665-9675. https://doi.org/10.2147/IJN.S215717