The discovery of novel protein-coding features in mouse genome based on mass spectrometry data

Xiao Bin Xing, Qing Run Li, Han Sun, Xing Fu, Fei Zhan, Xiu Huang, Jing Li, Chun Lei Chen, Yu Shyr, Rong Zeng, Yi Xue Li, Lu Xie

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

Identifying protein-coding genes in eukaryotic genomes remains a challenge in post-genome era due to the complex gene models. We applied a proteogenomics strategy to detect un-annotated protein-coding regions in mouse genome. High-accuracy tandem mass spectrometry (MS/MS) data from diverse mouse samples were generated by LTQ-Orbitrap mass spectrometer in house. Two searchable diagnostic proteomic datasets were constructed, one with all possible encoding exon junctions, and the other with all putative encoding exons, for the discovery of novel exon splicing events and novel uninterrupted protein-coding regions. Altogether 29,586 unique peptides were identified. Aligning backwards to the mouse genome, the translation of 4471 annotated genes was validated by the known peptides; and 172 genic events were defined in mouse genome by the novel peptides. The approach in the current work can provide substantial evidences for eukaryote genome annotation in encoding genes.

原文English
頁(從 - 到)343-351
頁數9
期刊Genomics
98
發行號5
DOIs
出版狀態Published - 2011 十一月

All Science Journal Classification (ASJC) codes

  • 遺傳學

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