TY - JOUR
T1 - The distribution of interleukin-19 in healthy and neoplastic tissue
AU - Hsing, Chung Hsi
AU - Li, Hsing Hui
AU - Hsu, Yu Hsiang
AU - Ho, Chung Liang
AU - Chuang, Shih Sung
AU - Lan, Kuo Mao
AU - Chang, Ming Shi
N1 - Funding Information:
We thank Mr. Tun-Yi Mou, Department of Physics, National Cheng Kung University, for building our arrayer. We also thank the Center of Excellence for Clinical Trial and Research in Oncology Specialty (supported by grant DOH-TD-B-111-004 from Department of Health, Taiwan) for providing the tissue arrayer service.
PY - 2008/11
Y1 - 2008/11
N2 - The influence of interleukin (IL)-19, a recently discovered cytokine in the IL-10 family, on tissue is still unclear. Our aim was to determine the distribution of IL-19 expression and to delineate the cell types that express IL-19 in healthy and neoplastic tissue, because this information will significantly facilitate the exploration of its pathophysiological functions. We used tissue microarray technology and an immunohistochemical survey with an anti-IL-19 monoclonal antibody to examine the expression of IL-19 in 28 healthy and 15 neoplastic tissues. IL-19 protein was positively stained in 15 healthy tissue types and three major cell types: epithelial cells, endothelial cells, and macrophages. We also found that several types of tumor cells were positively stained for IL-19, especially in squamous cell carcinoma (SCC) of the skin, tongue, esophagus, and lung. SCC of the oral cavity expressed IL-19 mRNA and its receptors. In two cell lines derived from SCC of oral cavity tumor tissue, IL-19 specifically activated an intracellular signal and induced proliferation of the cells, which indicated that IL-19 may act in an autocrine manner in SCC tumors. This study provides important references for further investigation of the biological functions and clinical implications of IL-19 in humans.
AB - The influence of interleukin (IL)-19, a recently discovered cytokine in the IL-10 family, on tissue is still unclear. Our aim was to determine the distribution of IL-19 expression and to delineate the cell types that express IL-19 in healthy and neoplastic tissue, because this information will significantly facilitate the exploration of its pathophysiological functions. We used tissue microarray technology and an immunohistochemical survey with an anti-IL-19 monoclonal antibody to examine the expression of IL-19 in 28 healthy and 15 neoplastic tissues. IL-19 protein was positively stained in 15 healthy tissue types and three major cell types: epithelial cells, endothelial cells, and macrophages. We also found that several types of tumor cells were positively stained for IL-19, especially in squamous cell carcinoma (SCC) of the skin, tongue, esophagus, and lung. SCC of the oral cavity expressed IL-19 mRNA and its receptors. In two cell lines derived from SCC of oral cavity tumor tissue, IL-19 specifically activated an intracellular signal and induced proliferation of the cells, which indicated that IL-19 may act in an autocrine manner in SCC tumors. This study provides important references for further investigation of the biological functions and clinical implications of IL-19 in humans.
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U2 - 10.1016/j.cyto.2008.06.007
DO - 10.1016/j.cyto.2008.06.007
M3 - Article
C2 - 18809337
AN - SCOPUS:55749090630
SN - 1043-4666
VL - 44
SP - 221
EP - 228
JO - Cytokine
JF - Cytokine
IS - 2
ER -