The dying process and cell viability in squamous cell carcinoma after arterial infusion of methotrexate: A light, ultrastructural, histochemical and enzyme analysis

M. C. Sheen, C. Y. Chai, T. M. Lin, C. F. Wu, Y. W. Wang, H. M. Sheu

研究成果: Article

摘要

Two fundamentally different processes of cell death, necrosis and apoptosis, are recognized recently. The nature of cancer cell death and cell kinetics after continuous intra-arterial infusion of methotrexate (MTX) were evaluated in a 47-year old patient with 3 huge, fungating squamous cell carcinoma over left lower limb. Six hours after initiation of external iliac arterial infusion, mitochondria swelling with rupture of internal cristae was the initial morphological changes under electron microscopic observation, followed by cell swelling, destruction of cytoplasmic organelles within 1-2 days, and finally fragmentation of nucleus of cancer cells accompanied by gross tissue inflammation. These morphological changes revealed the necrotic cell death in our case. Cell viability detected by an enzyme-histochemical staining of NADH-diaphorase demonstrated extensive cancer cell death 36 hours after therapy. During the remission period after therapy, residual cancer cells showed NADH-diaphorase positive and PCNA-cyclin negative staining, indicating that these viable cancer cells were in a non-proliferative state. In conclusion, continuous intra-arterial infusion of MTX induces a very rapid necrotic cell death in squamous cell carcinoma. A concurrent toxicity of MTX to the mitochondria may play a crucial role and act synergistically with inhibition of DNA synthesis to provoke cancer cell death.

原文English
頁(從 - 到)92-99
頁數8
期刊Regional Cancer Treatment
9
發行號2
出版狀態Published - 1996 十二月 1

指紋

Methotrexate
Squamous Cell Carcinoma
Cell Survival
Cell Death
Light
Enzymes
Dihydrolipoamide Dehydrogenase
Intra Arterial Infusions
Neoplasms
Mitochondria
Negative Staining
Cyclins
Proliferating Cell Nuclear Antigen
Residual Neoplasm
Cell Nucleus
Organelles
Rupture
Lower Extremity
Necrosis
Electrons

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pharmacology

引用此文

@article{77557687408141d784e89dd85fbd92c2,
title = "The dying process and cell viability in squamous cell carcinoma after arterial infusion of methotrexate: A light, ultrastructural, histochemical and enzyme analysis",
abstract = "Two fundamentally different processes of cell death, necrosis and apoptosis, are recognized recently. The nature of cancer cell death and cell kinetics after continuous intra-arterial infusion of methotrexate (MTX) were evaluated in a 47-year old patient with 3 huge, fungating squamous cell carcinoma over left lower limb. Six hours after initiation of external iliac arterial infusion, mitochondria swelling with rupture of internal cristae was the initial morphological changes under electron microscopic observation, followed by cell swelling, destruction of cytoplasmic organelles within 1-2 days, and finally fragmentation of nucleus of cancer cells accompanied by gross tissue inflammation. These morphological changes revealed the necrotic cell death in our case. Cell viability detected by an enzyme-histochemical staining of NADH-diaphorase demonstrated extensive cancer cell death 36 hours after therapy. During the remission period after therapy, residual cancer cells showed NADH-diaphorase positive and PCNA-cyclin negative staining, indicating that these viable cancer cells were in a non-proliferative state. In conclusion, continuous intra-arterial infusion of MTX induces a very rapid necrotic cell death in squamous cell carcinoma. A concurrent toxicity of MTX to the mitochondria may play a crucial role and act synergistically with inhibition of DNA synthesis to provoke cancer cell death.",
author = "Sheen, {M. C.} and Chai, {C. Y.} and Lin, {T. M.} and Wu, {C. F.} and Wang, {Y. W.} and Sheu, {H. M.}",
year = "1996",
month = "12",
day = "1",
language = "English",
volume = "9",
pages = "92--99",
journal = "Regional Cancer Treatment",
issn = "0935-0411",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - The dying process and cell viability in squamous cell carcinoma after arterial infusion of methotrexate

T2 - A light, ultrastructural, histochemical and enzyme analysis

AU - Sheen, M. C.

AU - Chai, C. Y.

AU - Lin, T. M.

AU - Wu, C. F.

AU - Wang, Y. W.

AU - Sheu, H. M.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Two fundamentally different processes of cell death, necrosis and apoptosis, are recognized recently. The nature of cancer cell death and cell kinetics after continuous intra-arterial infusion of methotrexate (MTX) were evaluated in a 47-year old patient with 3 huge, fungating squamous cell carcinoma over left lower limb. Six hours after initiation of external iliac arterial infusion, mitochondria swelling with rupture of internal cristae was the initial morphological changes under electron microscopic observation, followed by cell swelling, destruction of cytoplasmic organelles within 1-2 days, and finally fragmentation of nucleus of cancer cells accompanied by gross tissue inflammation. These morphological changes revealed the necrotic cell death in our case. Cell viability detected by an enzyme-histochemical staining of NADH-diaphorase demonstrated extensive cancer cell death 36 hours after therapy. During the remission period after therapy, residual cancer cells showed NADH-diaphorase positive and PCNA-cyclin negative staining, indicating that these viable cancer cells were in a non-proliferative state. In conclusion, continuous intra-arterial infusion of MTX induces a very rapid necrotic cell death in squamous cell carcinoma. A concurrent toxicity of MTX to the mitochondria may play a crucial role and act synergistically with inhibition of DNA synthesis to provoke cancer cell death.

AB - Two fundamentally different processes of cell death, necrosis and apoptosis, are recognized recently. The nature of cancer cell death and cell kinetics after continuous intra-arterial infusion of methotrexate (MTX) were evaluated in a 47-year old patient with 3 huge, fungating squamous cell carcinoma over left lower limb. Six hours after initiation of external iliac arterial infusion, mitochondria swelling with rupture of internal cristae was the initial morphological changes under electron microscopic observation, followed by cell swelling, destruction of cytoplasmic organelles within 1-2 days, and finally fragmentation of nucleus of cancer cells accompanied by gross tissue inflammation. These morphological changes revealed the necrotic cell death in our case. Cell viability detected by an enzyme-histochemical staining of NADH-diaphorase demonstrated extensive cancer cell death 36 hours after therapy. During the remission period after therapy, residual cancer cells showed NADH-diaphorase positive and PCNA-cyclin negative staining, indicating that these viable cancer cells were in a non-proliferative state. In conclusion, continuous intra-arterial infusion of MTX induces a very rapid necrotic cell death in squamous cell carcinoma. A concurrent toxicity of MTX to the mitochondria may play a crucial role and act synergistically with inhibition of DNA synthesis to provoke cancer cell death.

UR - http://www.scopus.com/inward/record.url?scp=0030404415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030404415&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030404415

VL - 9

SP - 92

EP - 99

JO - Regional Cancer Treatment

JF - Regional Cancer Treatment

SN - 0935-0411

IS - 2

ER -