The dynamic envelope of a fusion class II virus: E3 domain of glycoprotein E2 precursor in semliki forest virus provides a unique contact with the fusion protein E1

Shang Rung Wu, Lars Haag, Mathilda Sjöberg, Henrik Garoff, Lena Hammar

研究成果: Article

7 引文 (Scopus)

摘要

In alphaviruses, here represented by Semliki Forest virus, infection requires an acid-responsive spike configuration to facilitate membrane fusion. The creation of this relies on the chaperon function of glycoprotein E2 precursor (p62) and its maturation cleavage into the small external E3 and the membrane-anchored E2 glycoproteins. To reveal how the E3 domain of p62 exerts its control of spike functions, we determine the structure of a p62 cleavage-impaired mutant virus particle (SQL) by electron cryomicroscopy. A comparison with the earlier solved wild type virus structure reveals that the E3 domain of p62SQL forms a bulky side protrusion in the spike head region. This establishes a gripper over part of domain II of the fusion protein, with a cotter-like connection downward to a hydrophobic cluster in its central β-sheet. This finding reevaluates the role of the precursor from being only a provider of a shield over the fusion loop to a structural playmate in formation of the fusogenic architecture.

原文English
頁(從 - 到)26452-26460
頁數9
期刊Journal of Biological Chemistry
283
發行號39
DOIs
出版狀態Published - 2008 九月 26

指紋

Semliki forest virus
Viruses
Glycoproteins
Fusion reactions
Alphavirus
Cryoelectron Microscopy
Viral Structures
Membrane Fusion
Virus Diseases
Virion
Membranes
Proteins
Grippers
Head
Acids
Electrons

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

引用此文

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The dynamic envelope of a fusion class II virus : E3 domain of glycoprotein E2 precursor in semliki forest virus provides a unique contact with the fusion protein E1. / Wu, Shang Rung; Haag, Lars; Sjöberg, Mathilda; Garoff, Henrik; Hammar, Lena.

於: Journal of Biological Chemistry, 卷 283, 編號 39, 26.09.2008, p. 26452-26460.

研究成果: Article

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