The effect of immunotherapy on interleukin-1 and tumor necrosis factor production of monocytes in asthmatic children

Jiu Yao Wang, Huan Yao Lei, Kue Hsiung Hsieh

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

A longitudinal study was conducted to determine the pathogenesis and effect of immunotherapy (IT) on monocyte function. Production of interleukin-1 (IL-1) and tumor necrosis factor (TNF) by peripheral blood monocytes in 31 asthmatic children before and one year after IT was compared. Twenty-two children completed the treatment course, and 13 age-matched healthy children served as controls. Adherent monocytes were isolated and stimulated with either crude mite extract of Dermato-phagoid farinae (Df) for 7 days or lipopolysaccharide (LPS) for 3 days. The amount of TNF and IL-1 in culture supernatant was quantified by TNF and IL-1 enzyme-linked immunosorbent assay (ELISA) kits, respectively. The LPS-stimulated TNF production in patients was not different before or after FT (245.8 ± 110.9 vs. 213.3 ± 161.6 pg/0.1 ml, p ± 0.202), but was significantly higher than the control (66.7 ± 42.7 pg/0.1 ml; p < 0.0001). The LPS-stimulated IL-1 production was similar among the three groups. When stimulated with Of antigen, monocytes from asthmatic patients produced a greater amount of TNF and IL-1 than did those from the control (p < 0.001). Furthermore, although the production of TNF decreased after successful IT (360.2 ± 181.6 vs 243.9 ± 189.1 pg/0.1 ml, p < 0.05), the production of IL-1 did not change (679.9 ± 254.1 vs. 534.8 ± 257.6 pg/0.1 ml, p ± 0.05).Thus, repeated long-term administration of allergen (IT) was able to suppress specifically the TNF, but not IL-1 production of monocytes. The different results obtained from the stimulation by TNF by Dermatophagoid farinae after IT suggest the role of TNF on the hyposensitized state of monocytes after long-term, repeated injections of allergen.

原文English
頁(從 - 到)193-201
頁數9
期刊Journal of Asthma
29
發行號3
DOIs
出版狀態Published - 1992

All Science Journal Classification (ASJC) codes

  • 兒科、圍產兒和兒童健康
  • 免疫學和過敏
  • 肺和呼吸系統醫學

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