The effect of pH on the production of biohydrogen by clostridia: Thermodynamic and metabolic considerations

I. Chun Liu, Liang Ming Whang, Wei Jie Ren, Pei Ying Lin

研究成果: Article同行評審

98 引文 斯高帕斯(Scopus)

摘要

This study evaluates the effect of pH (4-7) on fermentative biohydrogen production by utilizing three isolated Clostridium species. Fermentative batch experiments show that the maximum hydrogen yield for Clostridium butyricum CGS2 (1.77 mmol/mmol glucose) is achieved at pH 6, whereas a high hydrogen production with Clostridium beijerinckii L9 (1.72 mmol/mmol glucose) and Clostridium tyrobutyricum FYa102 (1.83 mmol/mmol glucose) could be achieved under uncontrolled pH conditions (initial pH of 6.4-6.6 and final pH of 4-4.2). Low hydrogen yields (0-0.6 mmol/mmol glucose) observed at pH 4 are due likely to inhibitory effects on the microbial growth, although a low pH can be thermodynamically favorable for hydrogen production. The low hydrogen yields (0.12-0.64 mmol/mmol glucose) observed at pH 7 are attributed not only to thermodynamically unfavorable, but also metabolically unfavorable for hydrogen production. The relatively high levels of lactate, propionate, or formate observed at pH 7 reflect presumably the high enzymatic activities responsible for their production, together with the low hydrogenase activity, resulting in a low hydrogen production. A correlation analysis of the data from present and previous studies on biohydrogen production with pure Clostridium cultures and mixed microflora indicates a close relation between the hydrogen yield (YH2) and the (YH2)/(2(YHAc+YHBu)) ratio, with the observed correlation coefficient (0.787) higher than that (0.175) between YH2 and the molar ratio of butyrate to acetate (B/A). Based on the (YH2)/(2(YHAc+YHBu)) ratios observed at different pHs, a control of pH at 5.5-6.8 would seem to be an effective means to enhance the fermentative biohydrogen production.

原文English
頁(從 - 到)439-449
頁數11
期刊International Journal of Hydrogen Energy
36
發行號1
DOIs
出版狀態Published - 2011 1月

All Science Journal Classification (ASJC) codes

  • 可再生能源、永續發展與環境
  • 燃料技術
  • 凝聚態物理學
  • 能源工程與電力技術

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