The effect of velocity and extensional strain rate on enhancing DNA hybridization

Yung Chiang Chung, Yu Cheng Lin, Yuh Lih Hsu, Wu Ning Tommy Chang, Ming Zheng Shiu

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

A study of the effect of fluidic velocities and extensional strain rates on DNA hybridization microchips was conducted. The hybridization efficiency could be improved by introducing velocity and extensional strain rate. Compared with conventional hybridization methods, this microchip was able to increase the hybridization signal nine-fold within 30 min. Three different devices were designed, fabricated and tested using 1.4 kb single stranded DNA as the target. Excellent correlation between simulation analysis and experimental data was obtained. Experimental results showed that the effect of extensional strain rate on the hybridization was larger than that of velocity. Based on this information, a new design of hybridization chip with microfluidic concepts of velocity and extensional strain rate may provide additional efficiency in DNA detection. This hybridization microchip can provide potential applications in genomic study in the future.

原文English
頁(從 - 到)1376-1383
頁數8
期刊Journal of Micromechanics and Microengineering
14
發行號10
DOIs
出版狀態Published - 2004 10月 1

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 材料力學
  • 機械工業
  • 電氣與電子工程

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