TY - JOUR
T1 - The effect of velocity and extensional strain rate on enhancing DNA hybridization
AU - Chung, Yung Chiang
AU - Lin, Yu Cheng
AU - Hsu, Yuh Lih
AU - Chang, Wu Ning Tommy
AU - Shiu, Ming Zheng
PY - 2004/10/1
Y1 - 2004/10/1
N2 - A study of the effect of fluidic velocities and extensional strain rates on DNA hybridization microchips was conducted. The hybridization efficiency could be improved by introducing velocity and extensional strain rate. Compared with conventional hybridization methods, this microchip was able to increase the hybridization signal nine-fold within 30 min. Three different devices were designed, fabricated and tested using 1.4 kb single stranded DNA as the target. Excellent correlation between simulation analysis and experimental data was obtained. Experimental results showed that the effect of extensional strain rate on the hybridization was larger than that of velocity. Based on this information, a new design of hybridization chip with microfluidic concepts of velocity and extensional strain rate may provide additional efficiency in DNA detection. This hybridization microchip can provide potential applications in genomic study in the future.
AB - A study of the effect of fluidic velocities and extensional strain rates on DNA hybridization microchips was conducted. The hybridization efficiency could be improved by introducing velocity and extensional strain rate. Compared with conventional hybridization methods, this microchip was able to increase the hybridization signal nine-fold within 30 min. Three different devices were designed, fabricated and tested using 1.4 kb single stranded DNA as the target. Excellent correlation between simulation analysis and experimental data was obtained. Experimental results showed that the effect of extensional strain rate on the hybridization was larger than that of velocity. Based on this information, a new design of hybridization chip with microfluidic concepts of velocity and extensional strain rate may provide additional efficiency in DNA detection. This hybridization microchip can provide potential applications in genomic study in the future.
UR - http://www.scopus.com/inward/record.url?scp=7044222923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7044222923&partnerID=8YFLogxK
U2 - 10.1088/0960-1317/14/10/012
DO - 10.1088/0960-1317/14/10/012
M3 - Article
AN - SCOPUS:7044222923
SN - 0960-1317
VL - 14
SP - 1376
EP - 1383
JO - Journal of Micromechanics and Microengineering
JF - Journal of Micromechanics and Microengineering
IS - 10
ER -