The effects of magnetite (Fe3O4) nanoparticles on electroporation-induced inward currents in pituitary tumor (GH3) cells and in RAW 264.7 macrophages

研究成果: Article

10 引文 (Scopus)

摘要

Fe3O4 nanoparticles (NPs) have been known to provide a distinct image contrast effect for magnetic resonance imaging owing to their super paramagnetic properties on local magnetic fields. However, the possible effects of these NPs on membrane ion currents that concurrently induce local magnetic field perturbation remain unclear. Methods: We evaluated whether amine surface-modified Fe3O4 NPs have any effect on ion currents in pituitary tumor (GH3) cells via voltage clamp methods. Results: The addition of Fe3O4 NPs decreases the amplitude of membrane electroporationinduced currents (IMEP) with a half-maximal inhibitory concentration at 45 μg/mL. Fe3O4 NPs at a concentration of 3 mg/mL produced a biphasic response in the amplitude of IMEP, ie, an initial decrease followed by a sustained increase. A similar effect was also noted in RAW 264.7 macrophages. Conclusion: The modulation of magnetic electroporation-induced currents by Fe3O4 NPs constitutes an important approach for cell tracking under various imaging modalities or facilitated drug delivery.

原文English
頁(從 - 到)1687-1696
頁數10
期刊International journal of nanomedicine
7
DOIs
出版狀態Published - 2012 十二月 5

指紋

Magnetite Nanoparticles
Magnetite nanoparticles
Electroporation
Macrophages
Induced currents
Pituitary Neoplasms
Nanoparticles
Tumors
Magnetic Fields
Cell Tracking
Ions
Magnetic fields
Membranes
Imaging techniques
Clamping devices
Magnetic resonance
Drug delivery
Amines
RAW 264.7 Cells
Magnetic Resonance Imaging

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

引用此文

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title = "The effects of magnetite (Fe3O4) nanoparticles on electroporation-induced inward currents in pituitary tumor (GH3) cells and in RAW 264.7 macrophages",
abstract = "Fe3O4 nanoparticles (NPs) have been known to provide a distinct image contrast effect for magnetic resonance imaging owing to their super paramagnetic properties on local magnetic fields. However, the possible effects of these NPs on membrane ion currents that concurrently induce local magnetic field perturbation remain unclear. Methods: We evaluated whether amine surface-modified Fe3O4 NPs have any effect on ion currents in pituitary tumor (GH3) cells via voltage clamp methods. Results: The addition of Fe3O4 NPs decreases the amplitude of membrane electroporationinduced currents (IMEP) with a half-maximal inhibitory concentration at 45 μg/mL. Fe3O4 NPs at a concentration of 3 mg/mL produced a biphasic response in the amplitude of IMEP, ie, an initial decrease followed by a sustained increase. A similar effect was also noted in RAW 264.7 macrophages. Conclusion: The modulation of magnetic electroporation-induced currents by Fe3O4 NPs constitutes an important approach for cell tracking under various imaging modalities or facilitated drug delivery.",
author = "Liu, {Yen Chin} and Wu, {Ping Ching} and Shieh, {Dar Bin} and Wu, {Sheng Nan}",
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T1 - The effects of magnetite (Fe3O4) nanoparticles on electroporation-induced inward currents in pituitary tumor (GH3) cells and in RAW 264.7 macrophages

AU - Liu, Yen Chin

AU - Wu, Ping Ching

AU - Shieh, Dar Bin

AU - Wu, Sheng Nan

PY - 2012/12/5

Y1 - 2012/12/5

N2 - Fe3O4 nanoparticles (NPs) have been known to provide a distinct image contrast effect for magnetic resonance imaging owing to their super paramagnetic properties on local magnetic fields. However, the possible effects of these NPs on membrane ion currents that concurrently induce local magnetic field perturbation remain unclear. Methods: We evaluated whether amine surface-modified Fe3O4 NPs have any effect on ion currents in pituitary tumor (GH3) cells via voltage clamp methods. Results: The addition of Fe3O4 NPs decreases the amplitude of membrane electroporationinduced currents (IMEP) with a half-maximal inhibitory concentration at 45 μg/mL. Fe3O4 NPs at a concentration of 3 mg/mL produced a biphasic response in the amplitude of IMEP, ie, an initial decrease followed by a sustained increase. A similar effect was also noted in RAW 264.7 macrophages. Conclusion: The modulation of magnetic electroporation-induced currents by Fe3O4 NPs constitutes an important approach for cell tracking under various imaging modalities or facilitated drug delivery.

AB - Fe3O4 nanoparticles (NPs) have been known to provide a distinct image contrast effect for magnetic resonance imaging owing to their super paramagnetic properties on local magnetic fields. However, the possible effects of these NPs on membrane ion currents that concurrently induce local magnetic field perturbation remain unclear. Methods: We evaluated whether amine surface-modified Fe3O4 NPs have any effect on ion currents in pituitary tumor (GH3) cells via voltage clamp methods. Results: The addition of Fe3O4 NPs decreases the amplitude of membrane electroporationinduced currents (IMEP) with a half-maximal inhibitory concentration at 45 μg/mL. Fe3O4 NPs at a concentration of 3 mg/mL produced a biphasic response in the amplitude of IMEP, ie, an initial decrease followed by a sustained increase. A similar effect was also noted in RAW 264.7 macrophages. Conclusion: The modulation of magnetic electroporation-induced currents by Fe3O4 NPs constitutes an important approach for cell tracking under various imaging modalities or facilitated drug delivery.

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