The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan

Bo Yang Tsai, Chun Chih Chien, Shu Huan Huang, Jun Yuan Zheng, Chih Yu Hsu, Yau Sheng Tsai, Yuan Pin Hung, Wen Chien Ko, Pei Jane Tsai

研究成果: Article同行評審

摘要

Background: Several studies have highlighted the incidence of Clostridioides difficile infections (CDIs) in Taiwan and certain ribotypes have been related to severe clinical diseases. A study was conducted to investigate the polymerase chain reaction (PCR) ribotypes and genetic relatedness of clinical C. difficile strains collected from January 2009 to December 2015 at a hospital in northeastern Taiwan. Material and methods: A modified two-step typing algorithm for C. difficile was used by combining a modified 8-plex and 3′-truncated tcdA screening PCR. In addition, MLVA typing was adopted for investigation of bacterial clonality and transmission. Results: Among a total of 86 strains, 24 (28%) were nontoxigenic and 62 (72%) had both tcdA and tcdB (A + B+). No tcdA-negative and tcdB-positive (AB+) strains were identified. Binary toxin (CDT)-producing (cdtA+/cdtB+) strains were started to be identified in 2013. The 21 (34%) A+B+ clinical strains with binary toxin and tcdC deletion were identified as RT127 strains, which contained both RT078-lineage markers and fluoroquinolone (FQ)-resistant mutations (Thr82Ile in gyrA). Multiple loci variable-number tandem repeat analysis (MLVA) for phylogenetic relatedness of RT127 strains indicated that 20 of 21 strains belonged to a clonal complex that was identical to a clinical strain collected from southern Taiwan in 2011, suggestive of a clonal expansion in Taiwan. Conclusion: A two-step typing method could rapidly confirm species identification and define the toxin gene profile of C. difficile isolates. The clonal expansion of RT127 strains in Taiwan indicates monitoring and surveillance of toxigenic C. difficile isolates from human, animal, and environment are critical to develop One Health prevention strategies.

原文English
頁(從 - 到)896-909
頁數14
期刊Journal of Microbiology, Immunology and Infection
55
發行號5
DOIs
出版狀態Published - 2022 10月

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 免疫學與微生物學 (全部)
  • 微生物學(醫學)
  • 傳染性疾病

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