The expression and prognostic significance of hepatoma-derived growth factor in oral cancer

Yu Wei Lin, Chien Feng Li, Hsuan Yu Chen, Ching Yu Yen, Li Ching Lin, Chao Cheng Huang, Hsuan Ying Huang, Ping-Ching Wu, Chang Han Chen, San Cher Chen, Ming Hong Tai

研究成果: Article

21 引文 (Scopus)

摘要

Hepatoma-derived growth factor (HDGF) participates in oncogenic progression and represents a prognostic factor in several types of cancer. This study aimed to elucidate the role of HDGF during oral carcinogenesis. HDGF expression and the tumorigenic behaviors in human oral cell lines were investigated by immunoblotting, invasion and colony formation assays. Recombinant adenovirus vectors were employed to modulate the HDGF level in oral cancer cells. Immunohistochemical analysis using tissue microarray (TMA) consisting of surgically resected samples from 95 oral cancer patients was performed to delineate the correlation between HDGF expression and clinic-pathological parameters. HDGF expression was higher in malignant oral cancer cells than benign ones. Adenovirus-mediated HDGF overexpression and knockdown demonstrated the cellular HDGF level regulated the tumorigenic behaviors of oral cancer cells. Immunohistochemical analysis revealed increased HDGF expression in the nucleus and cytoplasm in oral cancer tissues. The nuclear HDGF expression was significantly correlated with tumor stage (P = 0.004) and grade (P = 0.013) while the cytoplasmic HDGF expression was associated with tumor necrosis (P = 0.002). Kaplan-Meier analysis revealed that patients with high nuclear HDGF expression had significantly worse 5-year disease-specific survival (P = 0.0069), metastasis-free survival (P = 0.0168), and local recurrence-free survival (P = 0.0047). Multivariate analysis indicated that the nuclear HDGF labeling index was an independent prognostic factor for disease-specific and local recurrence-free survival. HDGF overexpression contributes to the oncogenic processes in oral cancer cells and constitutes a novel prognostic factor for survival outcome of oral cancer patients.

原文English
頁(從 - 到)629-635
頁數7
期刊Oral Oncology
48
發行號7
DOIs
出版狀態Published - 2012 七月 1

指紋

Mouth Neoplasms
Survival
hepatoma-derived growth factor
Adenoviridae
Tissue Array Analysis
Recurrence
Neoplasms
Kaplan-Meier Estimate
Immunoblotting
Carcinogenesis
Cytoplasm
Necrosis

All Science Journal Classification (ASJC) codes

  • Oral Surgery
  • Oncology
  • Cancer Research

引用此文

Lin, Y. W., Li, C. F., Chen, H. Y., Yen, C. Y., Lin, L. C., Huang, C. C., ... Tai, M. H. (2012). The expression and prognostic significance of hepatoma-derived growth factor in oral cancer. Oral Oncology, 48(7), 629-635. https://doi.org/10.1016/j.oraloncology.2012.01.014
Lin, Yu Wei ; Li, Chien Feng ; Chen, Hsuan Yu ; Yen, Ching Yu ; Lin, Li Ching ; Huang, Chao Cheng ; Huang, Hsuan Ying ; Wu, Ping-Ching ; Chen, Chang Han ; Chen, San Cher ; Tai, Ming Hong. / The expression and prognostic significance of hepatoma-derived growth factor in oral cancer. 於: Oral Oncology. 2012 ; 卷 48, 編號 7. 頁 629-635.
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abstract = "Hepatoma-derived growth factor (HDGF) participates in oncogenic progression and represents a prognostic factor in several types of cancer. This study aimed to elucidate the role of HDGF during oral carcinogenesis. HDGF expression and the tumorigenic behaviors in human oral cell lines were investigated by immunoblotting, invasion and colony formation assays. Recombinant adenovirus vectors were employed to modulate the HDGF level in oral cancer cells. Immunohistochemical analysis using tissue microarray (TMA) consisting of surgically resected samples from 95 oral cancer patients was performed to delineate the correlation between HDGF expression and clinic-pathological parameters. HDGF expression was higher in malignant oral cancer cells than benign ones. Adenovirus-mediated HDGF overexpression and knockdown demonstrated the cellular HDGF level regulated the tumorigenic behaviors of oral cancer cells. Immunohistochemical analysis revealed increased HDGF expression in the nucleus and cytoplasm in oral cancer tissues. The nuclear HDGF expression was significantly correlated with tumor stage (P = 0.004) and grade (P = 0.013) while the cytoplasmic HDGF expression was associated with tumor necrosis (P = 0.002). Kaplan-Meier analysis revealed that patients with high nuclear HDGF expression had significantly worse 5-year disease-specific survival (P = 0.0069), metastasis-free survival (P = 0.0168), and local recurrence-free survival (P = 0.0047). Multivariate analysis indicated that the nuclear HDGF labeling index was an independent prognostic factor for disease-specific and local recurrence-free survival. HDGF overexpression contributes to the oncogenic processes in oral cancer cells and constitutes a novel prognostic factor for survival outcome of oral cancer patients.",
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Lin, YW, Li, CF, Chen, HY, Yen, CY, Lin, LC, Huang, CC, Huang, HY, Wu, P-C, Chen, CH, Chen, SC & Tai, MH 2012, 'The expression and prognostic significance of hepatoma-derived growth factor in oral cancer', Oral Oncology, 卷 48, 編號 7, 頁 629-635. https://doi.org/10.1016/j.oraloncology.2012.01.014

The expression and prognostic significance of hepatoma-derived growth factor in oral cancer. / Lin, Yu Wei; Li, Chien Feng; Chen, Hsuan Yu; Yen, Ching Yu; Lin, Li Ching; Huang, Chao Cheng; Huang, Hsuan Ying; Wu, Ping-Ching; Chen, Chang Han; Chen, San Cher; Tai, Ming Hong.

於: Oral Oncology, 卷 48, 編號 7, 01.07.2012, p. 629-635.

研究成果: Article

TY - JOUR

T1 - The expression and prognostic significance of hepatoma-derived growth factor in oral cancer

AU - Lin, Yu Wei

AU - Li, Chien Feng

AU - Chen, Hsuan Yu

AU - Yen, Ching Yu

AU - Lin, Li Ching

AU - Huang, Chao Cheng

AU - Huang, Hsuan Ying

AU - Wu, Ping-Ching

AU - Chen, Chang Han

AU - Chen, San Cher

AU - Tai, Ming Hong

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Hepatoma-derived growth factor (HDGF) participates in oncogenic progression and represents a prognostic factor in several types of cancer. This study aimed to elucidate the role of HDGF during oral carcinogenesis. HDGF expression and the tumorigenic behaviors in human oral cell lines were investigated by immunoblotting, invasion and colony formation assays. Recombinant adenovirus vectors were employed to modulate the HDGF level in oral cancer cells. Immunohistochemical analysis using tissue microarray (TMA) consisting of surgically resected samples from 95 oral cancer patients was performed to delineate the correlation between HDGF expression and clinic-pathological parameters. HDGF expression was higher in malignant oral cancer cells than benign ones. Adenovirus-mediated HDGF overexpression and knockdown demonstrated the cellular HDGF level regulated the tumorigenic behaviors of oral cancer cells. Immunohistochemical analysis revealed increased HDGF expression in the nucleus and cytoplasm in oral cancer tissues. The nuclear HDGF expression was significantly correlated with tumor stage (P = 0.004) and grade (P = 0.013) while the cytoplasmic HDGF expression was associated with tumor necrosis (P = 0.002). Kaplan-Meier analysis revealed that patients with high nuclear HDGF expression had significantly worse 5-year disease-specific survival (P = 0.0069), metastasis-free survival (P = 0.0168), and local recurrence-free survival (P = 0.0047). Multivariate analysis indicated that the nuclear HDGF labeling index was an independent prognostic factor for disease-specific and local recurrence-free survival. HDGF overexpression contributes to the oncogenic processes in oral cancer cells and constitutes a novel prognostic factor for survival outcome of oral cancer patients.

AB - Hepatoma-derived growth factor (HDGF) participates in oncogenic progression and represents a prognostic factor in several types of cancer. This study aimed to elucidate the role of HDGF during oral carcinogenesis. HDGF expression and the tumorigenic behaviors in human oral cell lines were investigated by immunoblotting, invasion and colony formation assays. Recombinant adenovirus vectors were employed to modulate the HDGF level in oral cancer cells. Immunohistochemical analysis using tissue microarray (TMA) consisting of surgically resected samples from 95 oral cancer patients was performed to delineate the correlation between HDGF expression and clinic-pathological parameters. HDGF expression was higher in malignant oral cancer cells than benign ones. Adenovirus-mediated HDGF overexpression and knockdown demonstrated the cellular HDGF level regulated the tumorigenic behaviors of oral cancer cells. Immunohistochemical analysis revealed increased HDGF expression in the nucleus and cytoplasm in oral cancer tissues. The nuclear HDGF expression was significantly correlated with tumor stage (P = 0.004) and grade (P = 0.013) while the cytoplasmic HDGF expression was associated with tumor necrosis (P = 0.002). Kaplan-Meier analysis revealed that patients with high nuclear HDGF expression had significantly worse 5-year disease-specific survival (P = 0.0069), metastasis-free survival (P = 0.0168), and local recurrence-free survival (P = 0.0047). Multivariate analysis indicated that the nuclear HDGF labeling index was an independent prognostic factor for disease-specific and local recurrence-free survival. HDGF overexpression contributes to the oncogenic processes in oral cancer cells and constitutes a novel prognostic factor for survival outcome of oral cancer patients.

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