The formation of oxidatively induced high-molecular-weight aggregate of α-/γ-crystallins

Fu Yung Huang, Chin Min Chia, Yuh Ho

研究成果: Article同行評審

7 引文 斯高帕斯(Scopus)

摘要

α-/γ-Crystallin interactions under oxidation with ascorbate-FeCl3-EDTA-H2O2 followed by dialysis have been studied. A high-molecular-weight aggregate (HMWA) composed of α- and γ-crystallin was observed for the mixture of the dialyzed α-crystallin and the oxidized γ-crystallin through gel-filtration chromatography. This illustrates an interaction between α-crystallin and partially denatured γ-crystallin induced by oxidation. No HMWA formation was observed under the condition without dialysis and/or with the addition of catalase to the oxidized γ-crystallin prior to the addition of α-crystallin. More HMWA was formed by oxidized γ-crystallin followed by the addition of α-crystallin than by simultaneous oxidation of both α- and γ-crystallins. Conformational changes of α-crystallin during oxidation analyzed by circular dichroism spectra showed that oxidized α-crystallin can gradually be restored to an ordered structure through dialysis. The overall results imply that structural changes of both α- and γ-crystallins and dialysis are required to form HMWA. The observation of this oxidatively induced chaperone/substrate complex suggests that an efficient chaperone-like protective action against oxidative insults may exist in vivo.

原文English
頁(從 - 到)60-65
頁數6
期刊Biochemical and Biophysical Research Communications
260
發行號1
DOIs
出版狀態Published - 1999 6月 24

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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