The highest fluorescence signal-to-noise ratio is achieved by optimizing the light acquisition direction and tube diameter of the QPCR system

Chia Lien Ma, Hsin Yi Tsai, Dar Bin Shieh, Kuo Cheng Huang, Chih Chung Yang, Yu Hsuan Lin

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

This study proposes an optical method to improve the yield efficiency of QPCR. The concept is that the conversion efficiency of fluorescent dyes is not high, and the light intensity signal is weak and difficult to distinguish. Therefore, the intensity signal of the excitation light source is reduced to obtain the fluorescent signal. Using the DNA binding dye as a model, the excitation light signals captured by the different angles between the incident light and the speculary reflected light are used to determine the correct fluorescent detection position, and to explore the efficiency of the test tube diameter for capturing fluorescent signals. The simulation results show that the standard diameter test tube has a minimum noise interference at the 45 degree position, which means that the fluorescent signal can be intercepted most effectively. This method does not change any chemical configuration and process conditions, saves a lot of time and process steps, and has high stability and zero pollution.

原文English
主出版物標題I2MTC 2020 - International Instrumentation and Measurement Technology Conference, Proceedings
發行者Institute of Electrical and Electronics Engineers Inc.
ISBN(電子)9781728144603
DOIs
出版狀態Published - 2020 五月
事件2020 IEEE International Instrumentation and Measurement Technology Conference, I2MTC 2020 - Dubrovnik, Croatia
持續時間: 2020 五月 252020 五月 29

出版系列

名字I2MTC 2020 - International Instrumentation and Measurement Technology Conference, Proceedings

Conference

Conference2020 IEEE International Instrumentation and Measurement Technology Conference, I2MTC 2020
國家/地區Croatia
城市Dubrovnik
期間20-05-2520-05-29

All Science Journal Classification (ASJC) codes

  • 安全、風險、可靠性和品質
  • 儀器

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