The influence of surface morphology and rigidity of the substrata on cell motility

Chien Chang Chen, Patrick Chin Ho Hsieh, Guo Ming Wang, Wen Chi Chen, Ming Long Yeh

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

The purpose of this study is to measure the cell motility under different stiffness and pattern of the substrata. Human melanoma cells were used for this study. Three surface patterns including flat, 6 μm-cone, and 6 μm-groove on polydimethylsiloxane (PDMS) were created by the micro-electro-mechanical system (MEMS). Glass dish was used as control substrate. After cells were seeded for 30 min, the cell images were taken every minute for 2 h. Each group had 4-5 dishes and 27-38 cells were calculated. The cell motility was 1.13 ± 1.30, 2.12 ± 1.21, 2.39 ± 1.11 and 3.08 ± 1.49 μm/min on glass, flat, cone, and groove PDMS, respectively. Cells in PDMS groups moved significantly faster than the control group (glass) due to smaller stiffness of the former substrates. More than 80% of cells on grooved PDMS moved along the grooves, indicating the grooved surface morphology could control the direction of cell movement. Our results display that substrate modulus and pattern can influence cell motility.

原文English
頁(從 - 到)1872-1875
頁數4
期刊Materials Letters
63
發行號21
DOIs
出版狀態Published - 2009 八月 31

All Science Journal Classification (ASJC) codes

  • 材料科學(全部)
  • 凝聚態物理學
  • 材料力學
  • 機械工業

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