The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts

Wenya Huang, William J. Feaver, Alan E. Tomkinson, Errol C. Friedberg

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.

原文English
頁(從 - 到)183-194
頁數12
期刊Mutation Research - DNA Repair
408
發行號3
DOIs
出版狀態Published - 1998 九月 11

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Toxicology
  • Genetics

指紋 深入研究「The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts」主題。共同形成了獨特的指紋。

引用此