Lineage mapping has identified both proliferative and quiescent intestinal stem cells, but the molecular circuitry controlling stem cell quiescence is incompletely understood. By lineage mapping, we show Lrig1, a pan-ErbB inhibitor, marks predominately noncycling, long-lived stem cells that are located at the crypt base and that, upon injury, proliferate and divide to replenish damaged crypts. Transcriptome profiling of Lrig1 + colonic stem cells differs markedly from the profiling of highly proliferative, Lgr5 + colonic stem cells; genes upregulated in the Lrig1 + population include those involved in cell cycle repression and response to oxidative damage. Loss of Apc in Lrig1 + cells leads to intestinal adenomas, and genetic ablation of Lrig1 results in heightened ErbB1-3 expression and duodenal adenomas. These results shed light on the relationship between proliferative and quiescent intestinal stem cells and support a model in which intestinal stem cell quiescence is maintained by calibrated ErbB signaling with loss of a negative regulator predisposing to neoplasia.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)