The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor

Wang Jing Liu, Chu Fang Lo, Guang Hsiung Kou, Jiann Horng Leu, Ying Jang Lai, Li Kwan Chang, Yun Shiang Chang

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

A series of deletion and mutation assays of the white spot syndrome virus (WSSV) immediate-early gene WSSV108 promoter showed that a Krüppel-like factor (KLF) binding site located from -504 to -495 (relative to the transcription start site) is important for the overall level of WSSV108 promoter activity. Electrophoretic mobility shift assays further showed that overexpressed recombinant Penaeus monodon KLF (rPmKLF) formed a specific protein-DNA complex with the 32P-labeled KLF binding site of the WSSV108 promoter, and that higher levels of Litopenaeus vannamei KLF (LvKLF) were expressed in WSSV-infected shrimp. A transactivation assay indicated that the WSSV108 promoter was strongly activated by rPmKLF in a dose-dependent manner. Lastly, we found that specific silencing of LvKLF expression in vivo by dsRNA injection dramatically reduced both WSSV108 expression and WSSV replication. We conclude that shrimp KLF is important for WSSV genome replication and gene expression, and that it binds to the WSSV108 promoter to enhance the expression of this immediate-early gene.

原文English
頁(從 - 到)7-18
頁數12
期刊Developmental and Comparative Immunology
49
發行號1
DOIs
出版狀態Published - 2015 三月 1

All Science Journal Classification (ASJC) codes

  • 免疫學
  • 發展生物學

指紋

深入研究「The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor」主題。共同形成了獨特的指紋。

引用此