The relationship between p53 status and anticancer drugs-induced apoptosis in nine human bladder cancer cell lines

Fu Lin Chang, Ming Derg Lai

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)

摘要

To study the relationship between the p53 status and chemotherapeutic drug-induced apoptosis, we have assessed the extent of apoptosis in nine bladder cancer cell lines during the treatment of adriamycin, cisplatin and methotrexate. Apoptosis was measured by the DNA fragmentation and merocyanine 540 (MC540) staining methods. Among the nine human bladder cancer cell lines, both wt-p53- and mut-p53-expressing cell lines (p53(+/-)) underwent apoptosis in response to anticancer drugs treatment. While the J82 (p53(-/-)) and TCCSUP (p53(+/+)) cell lines showed little or no apoptosis to these agents. Similar results were obtained when subjected to low doses of anticancer drug treatment. Interestingly, our results suggested that bladder cancer cells heterozygous for mutant p53 (+/-) seem to be most susceptible to chemotherapeutic drug. We therefore postulate that p53 mutations do not always provide a selective advantage in the development of chemoresistance, at least in bladderer tumor cell lines.

原文English
頁(從 - 到)351-355
頁數5
期刊Anticancer research
20
發行號1 A
出版狀態Published - 2000

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

指紋

深入研究「The relationship between p53 status and anticancer drugs-induced apoptosis in nine human bladder cancer cell lines」主題。共同形成了獨特的指紋。

引用此