Coming from the point of emergency room environment, this research studies the motion behavior, the control panel conformation, and the virtual operating movements of surgical nursing staff in the emergency room, in order to provide a reference for instrument design. The research began with professional interviews, followed by experiments on hand motion of surgical nursing staff in the emergency room. Test participants consist of surgical nursing staff from emergency rooms and the surgical department. A total of 20 participants, 10 females from each department were chosen to take the tests. The research is divided into three categories. Results of this study presented a difference in operation height range between different control panel directions amongst emergency room and non-emergency room surgical nursing staff. From the results we can see that participants with the same height do not necessary present the same wrist angles.