The role of RhoA kinase inhibition in human placenta-derived multipotent cells on neural phenotype and cell survival

Chih Hsiang Wang, Chia Ching Wu, Shan Hui Hsu, Jun Yang Liou, Yu Wei Li, Kenneth K. Wu, Yiu Kay Lai, B. Linju Yen

研究成果: Article同行評審

15 引文 斯高帕斯(Scopus)

摘要

Current advances in stem cell biology have brought much hope for therapy of neuro-degenerative diseases. However, neural stem cells (NSCs) are rare adult stem cells, and the use of non-NSCs requires efficient and high-yielding lineage-specific differentiation prior to transplantation for efficacy. We report on the efficient differentiation of placental-derived multipotent cells (PDMCs) into a neural phenotype with use of Y-27632, a clinically compliant small molecular inhibitor of Rho kinase (ROCK) which is a major mediator of cytoskeleton dynamics. Y-27632 does not induce differentiation of PDMC toward the mesodermal lineages of adipogenesis and osteogenesis, but rather a neural-like morphology, with rapid development of cell extensions and processes within 24 h. Compared with conventional neurogenic differentiation agents, Y-27632 induces a higher percentage of neural-like cells in PDMCs without arresting proliferation or cell cycle dynamics. Y-27632-treated PDMCs express several neural lineage genes at the RNA and protein level, including nestin, MAP2, and GFAP. The effect of the ROCK inhibitor is cell-specific to PDMCs, and is mainly mediated through the ROCK2 isoform and its downstream target, myosin II. Our data suggest that ROCK inhibition and cytoskeletal rearrangement may allow for induction of a neural phenotype in PDMCs without compromising cell survival.

原文English
頁(從 - 到)3223-3230
頁數8
期刊Biomaterials
34
發行號13
DOIs
出版狀態Published - 2013 4月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 陶瓷和複合材料
  • 生物物理學
  • 生物材料
  • 材料力學

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