TY - JOUR
T1 - The SEPT12 complex is required for the establishment of a functional sperm head-tail junction
AU - Shen, Yi Ru
AU - Wang, Han Yu
AU - Tsai, Yung Chieh
AU - Kuo, Yung Che
AU - Wu, Shang Rung
AU - Wang, Chia Yih
AU - Kuo, Pao Lin
N1 - Funding Information:
This work is supported by the Ministry of Science and Technology of Taiwan, R.O.C. (MOST 106-2314-B-006-056-MY3, 108-2811-B-006-509, 108-2811-B-006-518).
Funding Information:
The authors are grateful for the technical services provided by ‘Transgenic Mouse Model Core Facility of the National Core Facility for Biopharmaceuticals, Ministry of Science and Technology, Taiwan’ and the ‘Gene Knockout Mouse Core Laboratory of National Taiwan University Center of Genomic Medicine’. We would also like to acknowledge the support from the Core Research Laboratory, College of Medicine, National Cheng Kung University and the Laboratory Animal Center, Medical College, National Cheng Kung University.
Funding Information:
The authors are grateful for the technical services provided by ?Transgenic Mouse Model Core Facility of the National Core Facility for Biopharmaceuticals, Ministry of Science and Technology, Taiwan' and the ?Gene Knockout Mouse Core Laboratory of National Taiwan University Center of Genomic Medicine'. We would also like to acknowledge the support from the Core Research Laboratory, College of Medicine, National Cheng Kung University andthe Laboratory Animal Center, Medical College, National Cheng Kung University.
Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: [email protected].
PY - 2020/4/1
Y1 - 2020/4/1
N2 - The connecting pieces of the sperm neck link the flagellum and the sperm head, and they are important for initiating flagellar beating. The connecting pieces are important building blocks for the sperm neck; however, the mechanism of connecting piece assembly is poorly understood. In the present study, we explored the role of septins in sperm motility and found that Sept12D197N knock-in (KI) mice produce acephalic and immotile spermatozoa. Electron microscopy analysis showed defective connecting pieces in sperm from KI mice, indicating that SEPT12 is required for the establishment of connecting pieces. We also found that SEPT12 formed a complex with SEPT1, SEPT2, SEPT10 and SEPT11 at the sperm neck and that the D197N mutation disrupted the complex, suggesting that the SEPT12 complex is involved in the assembly of connecting pieces. Additionally, we found that SEPT12 interacted and colocalized with γ-tubulin in elongating spermatids, implying that SEPT12 and pericentriolar materials jointly contribute to the formation of connecting pieces. Collectively, our findings suggest that SEPT12 is required for the formation of striated columns, and the capitulum and for maintaining the stability of the sperm head-tail junction.
AB - The connecting pieces of the sperm neck link the flagellum and the sperm head, and they are important for initiating flagellar beating. The connecting pieces are important building blocks for the sperm neck; however, the mechanism of connecting piece assembly is poorly understood. In the present study, we explored the role of septins in sperm motility and found that Sept12D197N knock-in (KI) mice produce acephalic and immotile spermatozoa. Electron microscopy analysis showed defective connecting pieces in sperm from KI mice, indicating that SEPT12 is required for the establishment of connecting pieces. We also found that SEPT12 formed a complex with SEPT1, SEPT2, SEPT10 and SEPT11 at the sperm neck and that the D197N mutation disrupted the complex, suggesting that the SEPT12 complex is involved in the assembly of connecting pieces. Additionally, we found that SEPT12 interacted and colocalized with γ-tubulin in elongating spermatids, implying that SEPT12 and pericentriolar materials jointly contribute to the formation of connecting pieces. Collectively, our findings suggest that SEPT12 is required for the formation of striated columns, and the capitulum and for maintaining the stability of the sperm head-tail junction.
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U2 - 10.1093/molehr/gaaa031
DO - 10.1093/molehr/gaaa031
M3 - Article
C2 - 32392324
AN - SCOPUS:85087320318
SN - 1360-9947
VL - 26
SP - 402
EP - 412
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 6
ER -