The small subunit ribosomal RNA gene sequence of Pleistophora anguillarum and the use of PCR primers for diagnostic detection of the parasite

Hsu Wei Hung, Chu Fang Lo, Chieh Chih Tseng, Shao En Peng, Chih Ming Chou, Guang Hsiung Kou

研究成果: Article同行評審

15 引文 斯高帕斯(Scopus)

摘要

Using the polymerase chain reaction (PCR) and two primers for conserved regions of the small subunit ribosomal RNA (SSU-rRNA) of Microsporidia, a DNA segment about 1,195 base pairs long was amplified from a DNA template prepared from purified spores of the microsporidian species Pleistophora anguillarum. These spores had been isolated from adult eels (Anguilla japonica) with 'Beko Disease.' A comparison of sequence data from other microsporidian species showed P. anguillarum SSU-rRNA to be most similar to Vavraia oncoperae. When juvenile eels were artificially infected with P. anguillarum, enzyme-linked immunosorbent assay could detect a positive infection only 12 days post-infection. However, when suitable PCR primers were used, a DNA fragment of about 0.8 kb was detected from these juvenile eels after only 3 days post-infection. No PCR product was obtained with templates prepared from clinically healthy control animals.

原文English
頁(從 - 到)556-560
頁數5
期刊Journal of Eukaryotic Microbiology
45
發行號5
DOIs
出版狀態Published - 1998 一月 1

All Science Journal Classification (ASJC) codes

  • Microbiology

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