The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses

Zhen Zhang, Dongsheng Wang, Tao Sun, Jianhua Xu, Hsueh Cheng Chiang, Wonchul Shin, Ling Gang Wu

研究成果: Article同行評審

35 引文 斯高帕斯(Scopus)

摘要

SNAP25, an essential component of the soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex that mediates exocytosis, is not considered to play a role in endocytosis, which couples to exocytosis by retrieving a similar amount of exocytosed vesicles. By knocking down SNAP25 and imaging slow endocytosis at a conventional synapse, the rat cultured hippocampal synapse, we found that SNAP25 is involved in slow, clathrin-dependent endocytosis. With similar techniques, we found that not only SNAP25, but also synaptobrevin is involved in slow endocytosis. These results provide the first evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at conventional synapses. Such a dual role may contribute to mediate the coupling between exocytosis and clathrin-dependent endocytosis at conventional synapses, a mechanism critical for the maintenance of synaptic transmission and the normal structure of nerve terminals.

原文English
頁(從 - 到)9169-9175
頁數7
期刊Journal of Neuroscience
33
發行號21
DOIs
出版狀態Published - 2013 五月 22

All Science Journal Classification (ASJC) codes

  • 神經科學 (全部)

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