The thymidylate synthase gene of Hz-1 virus: A gene captured from its lepidopteran host

Hong Hwa Chen, Dan Ju Tso, Wen Bin Yeh, Hsien Jen Cheng, Ting Feng Wu

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus HindIII-D fragment. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR. Results of phylogenetic analysis based on non-synonymous and amino acid distances suggested that the TS gene of Hz-1 virus was grouped closely with that of Bombyx mori. High bootstrap values confirmed that the thymidylate synthase of Hz-1 virus was acquired by a capture event from its lepidopteran host. Results of both sequence divergences and phylogenetic analysis suggested that TS genes in insect viruses, Hz-1, CIV, and MsEPV may have a different history or originated from different capture events.

原文English
頁(從 - 到)495-503
頁數9
期刊Insect Molecular Biology
10
發行號5
DOIs
出版狀態Published - 2001

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學
  • 昆蟲科學

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