The trend of macrolide resistance and emm types of group A streptococci from children at a medical center in southern Taiwan

Po Kai Chuang, Shih-Min Wang, Hui Chen Lin, Yu Hao Cho, Yun Ju Ma, Tzong-Shiann Ho, Ching-Fen Shen, Ching-Chuan Liu

研究成果: Article

4 引文 (Scopus)

摘要

Group A streptococcus (GAS) is a common pathogen in children. Macrolide resistance in GAS has been described worldwide. The aims of this study are to analyze macrolide resistance of GAS isolates in southern Taiwan and to clarify the relationship of emm typing and macrolide resistance in the past decade. Methods: All GAS isolated from patients younger than 18 years at a single tertiary center in southern Taiwan were collected from 2000 to 2012. Antibiotics susceptibility to erythromycin, azithromycin, and clindamycin were determined by agar dilution method, and were interpreted by Clinical and Laboratory Standards Institute (CLSI) standards. emm typing was performed by polymerase chain reaction (PCR). Results: A total of 301 isolates were collected during the period of 13 years. Scarlet fever (38.5%) and acute pharyngitis (32.2%) were the most common diagnosis. Decreased resistance rate of erythromycin from 53.1% in 2000 to 0% in 2010 was found, but it increased rapidly to 65% in 2011. The resistance rate of azithromycin was the lowest (4.2%) in 2005, but was higher than 15% after 2006. The involvement of the erythromycin resistance genes were mefA (53.1%), ermB (35.9%), and ermTR (10.9%). The resistance of clindamycin also increased since 2011. emm12 was the most common serotype and accounted for 44.9% of all isolates. Compared with the non-. emm12 group, resistance to erythromycin, azithromycin, and clindamycin were more frequently detected in the emm12 group. Conclusion: Increased resistance of GAS to macrolide and clindamycin was found in recent years. emm12 was the main serotype for macrolide resistance.

原文English
頁(從 - 到)160-167
頁數8
期刊Journal of Microbiology, Immunology and Infection
48
發行號2
DOIs
出版狀態Published - 2015 四月 1

指紋

Macrolides
Streptococcus
Taiwan
Clindamycin
Erythromycin
Azithromycin
Scarlet Fever
Pharyngitis
Agar
Anti-Bacterial Agents
Polymerase Chain Reaction
Genes
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Medicine(all)

引用此文

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title = "The trend of macrolide resistance and emm types of group A streptococci from children at a medical center in southern Taiwan",
abstract = "Group A streptococcus (GAS) is a common pathogen in children. Macrolide resistance in GAS has been described worldwide. The aims of this study are to analyze macrolide resistance of GAS isolates in southern Taiwan and to clarify the relationship of emm typing and macrolide resistance in the past decade. Methods: All GAS isolated from patients younger than 18 years at a single tertiary center in southern Taiwan were collected from 2000 to 2012. Antibiotics susceptibility to erythromycin, azithromycin, and clindamycin were determined by agar dilution method, and were interpreted by Clinical and Laboratory Standards Institute (CLSI) standards. emm typing was performed by polymerase chain reaction (PCR). Results: A total of 301 isolates were collected during the period of 13 years. Scarlet fever (38.5{\%}) and acute pharyngitis (32.2{\%}) were the most common diagnosis. Decreased resistance rate of erythromycin from 53.1{\%} in 2000 to 0{\%} in 2010 was found, but it increased rapidly to 65{\%} in 2011. The resistance rate of azithromycin was the lowest (4.2{\%}) in 2005, but was higher than 15{\%} after 2006. The involvement of the erythromycin resistance genes were mefA (53.1{\%}), ermB (35.9{\%}), and ermTR (10.9{\%}). The resistance of clindamycin also increased since 2011. emm12 was the most common serotype and accounted for 44.9{\%} of all isolates. Compared with the non-. emm12 group, resistance to erythromycin, azithromycin, and clindamycin were more frequently detected in the emm12 group. Conclusion: Increased resistance of GAS to macrolide and clindamycin was found in recent years. emm12 was the main serotype for macrolide resistance.",
author = "Chuang, {Po Kai} and Shih-Min Wang and Lin, {Hui Chen} and Cho, {Yu Hao} and Ma, {Yun Ju} and Tzong-Shiann Ho and Ching-Fen Shen and Ching-Chuan Liu",
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TY - JOUR

T1 - The trend of macrolide resistance and emm types of group A streptococci from children at a medical center in southern Taiwan

AU - Chuang, Po Kai

AU - Wang, Shih-Min

AU - Lin, Hui Chen

AU - Cho, Yu Hao

AU - Ma, Yun Ju

AU - Ho, Tzong-Shiann

AU - Shen, Ching-Fen

AU - Liu, Ching-Chuan

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Group A streptococcus (GAS) is a common pathogen in children. Macrolide resistance in GAS has been described worldwide. The aims of this study are to analyze macrolide resistance of GAS isolates in southern Taiwan and to clarify the relationship of emm typing and macrolide resistance in the past decade. Methods: All GAS isolated from patients younger than 18 years at a single tertiary center in southern Taiwan were collected from 2000 to 2012. Antibiotics susceptibility to erythromycin, azithromycin, and clindamycin were determined by agar dilution method, and were interpreted by Clinical and Laboratory Standards Institute (CLSI) standards. emm typing was performed by polymerase chain reaction (PCR). Results: A total of 301 isolates were collected during the period of 13 years. Scarlet fever (38.5%) and acute pharyngitis (32.2%) were the most common diagnosis. Decreased resistance rate of erythromycin from 53.1% in 2000 to 0% in 2010 was found, but it increased rapidly to 65% in 2011. The resistance rate of azithromycin was the lowest (4.2%) in 2005, but was higher than 15% after 2006. The involvement of the erythromycin resistance genes were mefA (53.1%), ermB (35.9%), and ermTR (10.9%). The resistance of clindamycin also increased since 2011. emm12 was the most common serotype and accounted for 44.9% of all isolates. Compared with the non-. emm12 group, resistance to erythromycin, azithromycin, and clindamycin were more frequently detected in the emm12 group. Conclusion: Increased resistance of GAS to macrolide and clindamycin was found in recent years. emm12 was the main serotype for macrolide resistance.

AB - Group A streptococcus (GAS) is a common pathogen in children. Macrolide resistance in GAS has been described worldwide. The aims of this study are to analyze macrolide resistance of GAS isolates in southern Taiwan and to clarify the relationship of emm typing and macrolide resistance in the past decade. Methods: All GAS isolated from patients younger than 18 years at a single tertiary center in southern Taiwan were collected from 2000 to 2012. Antibiotics susceptibility to erythromycin, azithromycin, and clindamycin were determined by agar dilution method, and were interpreted by Clinical and Laboratory Standards Institute (CLSI) standards. emm typing was performed by polymerase chain reaction (PCR). Results: A total of 301 isolates were collected during the period of 13 years. Scarlet fever (38.5%) and acute pharyngitis (32.2%) were the most common diagnosis. Decreased resistance rate of erythromycin from 53.1% in 2000 to 0% in 2010 was found, but it increased rapidly to 65% in 2011. The resistance rate of azithromycin was the lowest (4.2%) in 2005, but was higher than 15% after 2006. The involvement of the erythromycin resistance genes were mefA (53.1%), ermB (35.9%), and ermTR (10.9%). The resistance of clindamycin also increased since 2011. emm12 was the most common serotype and accounted for 44.9% of all isolates. Compared with the non-. emm12 group, resistance to erythromycin, azithromycin, and clindamycin were more frequently detected in the emm12 group. Conclusion: Increased resistance of GAS to macrolide and clindamycin was found in recent years. emm12 was the main serotype for macrolide resistance.

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