@article{eadb6d5cf1de4a5a87936f7b43bb3f6c,
title = "Therapeutic potential of nanoceria pretreatment in preventing the development of urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and SerpinB2 downregulation",
abstract = "Urological chronic pelvic pain syndrome (UCPPS) manifests as pelvic pain with frequent urination and has a 10% prevalence rate without effective therapy. Nanoceria (cerium oxide nanoparticles [CNPs]) were synthesized in this study to achieve potential long-term pain relief, using a commonly used UCPPS mouse model with cyclophosphamide-induced cystitis. Transcriptome sequencing analysis revealed that serpin family B member 2 (SerpinB2) was the most upregulated marker in mouse bladder, and SerpinB2 was downregulated with CNP pretreatment. The transcriptome sequencing analysis results agreed with quantitative polymerase chain reaction and western blot analysis results for the expression of related mRNAs and proteins. Analysis of Gene Expression Omnibus (GEO) datasets revealed that SerpinB2 was a differentially upregulated gene in human UCPPS. In vitro SerpinB2 knockdown downregulated proinflammatory chemokine expression (chemokine receptor CXCR3 and C-X-C motif chemokine ligand 10) upon treatment with 4-hydroperoxycyclophosphamide. In conclusion, CNP pretreatment may prevent the development of UCPPS, and reactive oxygen species (ROS) scavenging and SerpinB2 downregulation may modulate the immune response in UCPPS.",
author = "Lien, {Wei Chih} and Zhou, {Xin Ran} and Liang, {Ya Jyun} and Ching, {Congo Tak Shing} and Wang, {Chia Yih} and Lu, {Fu I.} and Chang, {Huei Cih} and Lin, {Feng Huei} and Wang, {Hui Min David}",
note = "Funding Information: This study was supported by the Ministry of Science and Technology, Taiwan (grant numbers MOST110‐2314‐B‐006‐096 to Wei‐Chih Lien; MOST110‐2221‐E‐005‐010 to Hui‐Min David Wang); the National Cheng Kung University Hospital, Taiwan (grant numbers NCKUH‐10909013, NCKUH‐11004013, and NCKUH‐11102065 to Wei‐Chih Lien); the ENABLE project of the National Chung Hsing University (grant number ENABLE:110ST001G); and Kasetsart University Joint Research Project (grant number: 108RA129A). The funders played no role in the study design, data collection and analysis, interpretation of data, or writing of the manuscript. Funding Information: The authors are grateful to Miss Ping-Hsuan Chou and Associate Prof. Pei-Fang Su, Department of Statistics, College of Management, National Cheng Kung University, for providing statistical consulting services. Wei-Chih Lien carried out his thesis research under the auspices of the Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, and National Health Research Institutes. Publisher Copyright: {\textcopyright} 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.",
year = "2023",
month = jan,
doi = "10.1002/btm2.10346",
language = "English",
volume = "8",
journal = "Bioengineering and Translational Medicine",
issn = "2380-6761",
publisher = "John Wiley & Sons Inc.",
number = "1",
}