Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning

Heejin Choi, Dushan N. Wadduwage, Ting Yuan Tu, Paul Matsudaira, Peter T.C. So

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth-resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:105.

原文English
頁(從 - 到)49-60
頁數12
期刊Cytometry Part A
87
發行號1
DOIs
出版狀態Published - 2015 一月 1

All Science Journal Classification (ASJC) codes

  • 病理學與法醫學
  • 組織學
  • 細胞生物學

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