TY - JOUR
T1 - Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning
AU - Choi, Heejin
AU - Wadduwage, Dushan N.
AU - Tu, Ting Yuan
AU - Matsudaira, Paul
AU - So, Peter T.C.
N1 - Publisher Copyright:
© 2014 International Society for Advancement of Cytometry.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth-resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:105.
AB - A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth-resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:105.
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U2 - 10.1002/cyto.a.22584
DO - 10.1002/cyto.a.22584
M3 - Article
C2 - 25352187
AN - SCOPUS:84919668330
SN - 1552-4922
VL - 87
SP - 49
EP - 60
JO - Cytometry Part A
JF - Cytometry Part A
IS - 1
ER -