Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane

Chi Chou Chiu, Hsou Min Li

研究成果: Article同行評審

34 引文 斯高帕斯(Scopus)

摘要

Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.

原文English
頁(從 - 到)793-801
頁數9
期刊Plant Journal
56
發行號5
DOIs
出版狀態Published - 2008 十二月

All Science Journal Classification (ASJC) codes

  • 遺傳學
  • 植物科學
  • 細胞生物學

指紋

深入研究「Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane」主題。共同形成了獨特的指紋。

引用此