Tks5 and dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion

Mei Chun Chuang, Shan Shan Lin, Ryosuke L. Ohniwa, Gang Hui Lee, You An Su, Yu Chen Chang, Ming Jer Tang, Ya Wen Liu

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

Skeletal muscle development requires the cell–cell fusion of differentiated myoblasts to form muscle fibers. The actin cytoskeleton is known to be the main driving force for myoblast fusion; however, how actin is organized to direct intercellular fusion remains unclear. Here we show that an actin- and dynamin-2–enriched protrusive structure, the invadosome, is required for the fusion process of myogenesis. Upon differentiation, myoblasts acquire the ability to form invadosomes through isoform switching of a critical invadosome scaffold protein, Tks5. Tks5 directly interacts with and recruits dynamin-2 to the invadosome and regulates its assembly around actin filaments to strengthen the stiffness of dynamin-actin bundles and invadosomes. These findings provide a mechanistic framework for the acquisition of myogenic fusion machinery during myogenesis and reveal a novel structural function for Tks5 and dynamin-2 in organizing actin filaments in the invadosome to drive membrane fusion.

原文English
頁(從 - 到)1670-1685
頁數16
期刊Journal of Cell Biology
218
發行號5
DOIs
出版狀態Published - 2019 五月 1

All Science Journal Classification (ASJC) codes

  • 細胞生物學

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