Toxicity assessment of mono-substituted benzenes and phenols using a Pseudomonas initial oxygen uptake assay

Ded Shih Huang, Thou Jen Whang, Fei Chen Cheng, Ya Ping Wu, Yi Ting Wang, Wen I. Luo, Yane Shih Wang

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

A methodology is presented for assessing the toxicity of chemical substances through their inhibitory action toward the Pseudomonas initial oxygen uptake (PIOU) rate. The current studies reveal that the PIOU assay is rapid, cost-efficient, and easy to perform. The oxygen uptake rate was found to be associated with a putative benzoate transporter and highly dependent on benzoate concentration. The putative benzoate transporter has been shown to follow Michaelis-Menten kinetics. Most phenols were found to be noncompetitive inhibitors of the benzoate transporter. The inhibition constant (Ki) of these noncompetitive inhibitors can be related to the concentration causing 50% oxygen uptake inhibition in Pseudomonas putida. Modeling these data by using the response-surface approach leads to the development of a quantitative structure-activity relationship (QSAR) for the toxicity of phenols ((1/K i) = -0.435 (±0.038) lowest-unoccupied-molecular orbital + 0.517 (±0.027)log KOW - 2.340 (±0.068), n = 49, r 2 = 0.930, s = 0.107, radj2 = 0.926, F = 303.1). A comparison of QSAR models derived from the Ki data of the PIOU method and the toxicity data of 40-h Tetrahymena pyrifomis growth inhibition assay (Tetratox) indicated that there was a high correlation between the two approaches (r2 = 0.925).

原文English
頁(從 - 到)253-260
頁數8
期刊Environmental Toxicology and Chemistry
24
發行號2
DOIs
出版狀態Published - 2005 2月

All Science Journal Classification (ASJC) codes

  • 環境化學
  • 健康、毒理學和誘變

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