Transactivation of protein expression by rice HSP101 in planta and using Hsp101 as a selection marker for transformation

Ching Chun Chang, Pin Sheng Huang, Huei Ru Lin, Chun Hsien Lu

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36 引文 斯高帕斯(Scopus)

摘要

Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.

原文English
頁(從 - 到)1098-1107
頁數10
期刊Plant and Cell Physiology
48
發行號8
DOIs
出版狀態Published - 2007 8月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學

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