Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma

Jin Yuan Shih, Meng Feng Tsai, Tzu Hua Chang, Yih Leong Chang, Ang Yuan, Chong Jen Yu, Shin Bey Lin, Geou Yarh Liou, Meng Larn Lee, Jeremy J.W. Chen, Tse-Ming Hong, Shuenn Chen Yang, Jen Liang Su, Yung Chie Lee, Pan Chyr Yang

研究成果: Article

166 引文 (Scopus)

摘要

Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.

原文English
頁(從 - 到)8070-8078
頁數9
期刊Clinical Cancer Research
11
發行號22
DOIs
出版狀態Published - 2005 十一月 15

指紋

Gastropoda
Carcinoma
Matrix Metalloproteinase 2
Neoplasms
Adenocarcinoma of lung
Heterografts
Messenger RNA
Reverse Transcription
Cell Line
Polymerase Chain Reaction
Survival
Gene Expression Profiling
Cadherins
Microvessels

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

引用此文

Shih, J. Y., Tsai, M. F., Chang, T. H., Chang, Y. L., Yuan, A., Yu, C. J., ... Yang, P. C. (2005). Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma. Clinical Cancer Research, 11(22), 8070-8078. https://doi.org/10.1158/1078-0432.CCR-05-0687
Shih, Jin Yuan ; Tsai, Meng Feng ; Chang, Tzu Hua ; Chang, Yih Leong ; Yuan, Ang ; Yu, Chong Jen ; Lin, Shin Bey ; Liou, Geou Yarh ; Lee, Meng Larn ; Chen, Jeremy J.W. ; Hong, Tse-Ming ; Yang, Shuenn Chen ; Su, Jen Liang ; Lee, Yung Chie ; Yang, Pan Chyr. / Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma. 於: Clinical Cancer Research. 2005 ; 卷 11, 編號 22. 頁 8070-8078.
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title = "Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma",
abstract = "Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.",
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Shih, JY, Tsai, MF, Chang, TH, Chang, YL, Yuan, A, Yu, CJ, Lin, SB, Liou, GY, Lee, ML, Chen, JJW, Hong, T-M, Yang, SC, Su, JL, Lee, YC & Yang, PC 2005, 'Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma', Clinical Cancer Research, 卷 11, 編號 22, 頁 8070-8078. https://doi.org/10.1158/1078-0432.CCR-05-0687

Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma. / Shih, Jin Yuan; Tsai, Meng Feng; Chang, Tzu Hua; Chang, Yih Leong; Yuan, Ang; Yu, Chong Jen; Lin, Shin Bey; Liou, Geou Yarh; Lee, Meng Larn; Chen, Jeremy J.W.; Hong, Tse-Ming; Yang, Shuenn Chen; Su, Jen Liang; Lee, Yung Chie; Yang, Pan Chyr.

於: Clinical Cancer Research, 卷 11, 編號 22, 15.11.2005, p. 8070-8078.

研究成果: Article

TY - JOUR

T1 - Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma

AU - Shih, Jin Yuan

AU - Tsai, Meng Feng

AU - Chang, Tzu Hua

AU - Chang, Yih Leong

AU - Yuan, Ang

AU - Yu, Chong Jen

AU - Lin, Shin Bey

AU - Liou, Geou Yarh

AU - Lee, Meng Larn

AU - Chen, Jeremy J.W.

AU - Hong, Tse-Ming

AU - Yang, Shuenn Chen

AU - Su, Jen Liang

AU - Lee, Yung Chie

AU - Yang, Pan Chyr

PY - 2005/11/15

Y1 - 2005/11/15

N2 - Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.

AB - Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.

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