Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma

Jin Yuan Shih, Meng Feng Tsai, Tzu Hua Chang, Yih Leong Chang, Ang Yuan, Chong Jen Yu, Shin Bey Lin, Geou Yarh Liou, Meng Larn Lee, Jeremy J.W. Chen, Tse Ming Hong, Shuenn Chen Yang, Jen Liang Su, Yung Chie Lee, Pan Chyr Yang

研究成果: Article同行評審

177 引文 斯高帕斯(Scopus)

摘要

Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.

原文English
頁(從 - 到)8070-8078
頁數9
期刊Clinical Cancer Research
11
發行號22
DOIs
出版狀態Published - 2005 十一月 15

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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