Transcriptome analysis of age-related gain of callus-forming capacity in arabidopsis hypocotyls

Chi Chien Chen, Shih Feng Fu, Yung I. Lee, Chung Yi Lin, Wan Chen Lin, Hao Jen Huang

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)


Callus-forming capacity is enhanced with hypocotyl maturity in Arabidopsis. However, the genetic regulation of age-related gain in capacity for callus formation is unclear. We used a gene expression microarray assay to characterize the underlying mechanisms during callus formation in young and mature hypocotyl explants of Arabidopsis. As expected, genes involved in photosynthesis and cell wall thickening showed altered expression during hypocotyl maturation. In addition, genes involved in cytokinin perception were enriched in mature hypocotyl tissues. Phytohormone-induced callus formation in hypocotyl explants was accompanied by increased expression of genes mainly related to the cell cycle, histones and epigenetics. The induction level of these genes was higher in mature hypocotyl explants than young explants during callus formation. We identified a number of genes, including those with unknown function, potentially involved in age-related gain in callus formation. Our results provide insight into the effect of hypocotyl age on callus formation. Altered cytokinin signaling components, cell cycle regulation and epigenetics may work in concert to lead to gain of callus-forming capacity in hypocotyls with age.

頁(從 - 到)1457-1469
期刊Plant and Cell Physiology
出版狀態Published - 2012 八月

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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