Transcutaneous immunization of Streptococcus suis bacterin using dissolving microneedles

Kai Jen Hsueh, Mei Chin Chen, Li Ting Cheng, Jai Wei Lee, Wen Bin Chung, Chun Yen Chu

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)


Vaccine delivery using microneedle (MN) patches is an easy, safe and painless alternative to traditional needle injections. In this study, we examined whether MN patches can enhance the efficacy of a Streptococcus suis serotype 2 (S. suis 2) vaccine in a mouse model. Results showed that MNs can reach 200–250 μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. Vaccination with prime-boost of MN induced higher levels of IgG2a antibody titer, T cell proliferation, and TH1 cytokines (IFN-γ and IL-12) as compared to intramuscular (IM) injection. In addition, single dose MN vaccination better protected mice against lethal challenge than IM vaccination. MN vaccination also conferred long-term IgG2a antibody against S. suis 2 bacteria presence for up to 7 months. Taken together, these data showed that vaccine delivery by MNs results in superior immune response and protection rate when compared to IM injections.

頁(從 - 到)78-87
期刊Comparative Immunology, Microbiology and Infectious Diseases
出版狀態Published - 2017 二月 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

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