@article{8d473cc33a814424ac1bbc214b1639f1,
title = "Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer",
abstract = "By using RNA-immunoprecipitation assay following next-generation sequencing, a group of cell cycle-related genes targeted by hnRNP Q1 were identified, including Aurora-A kinase. Overexpressed hnRNP Q1 can upregulate Aurora-A protein, but not alter the mRNA level, through enhancing the translational efficiency of Aurora-A mRNA, either in a cap-dependent or -independent manner, by interacting with the 5′-UTR of Aurora-A mRNA through its RNA-binding domains (RBDs) 2 and 3. By ribosomal profiling assay further confirmed the translational regulation of Aurora-A mRNA by hnRNP Q1. Overexpression of hnRNP Q1 promotes cell proliferation and tumor growth. HnRNP Q1/ΔRBD23-truncated mutant, which loses the binding ability and translational regulation of Aurora-A mRNA, has no effect on promoting tumor growth. The expression level of hnRNP Q1 is positively correlated with Aurora-A in colorectal cancer. Taken together, our data indicate that hnRNP Q1 is a novel trans-acting factor that binds to Aurora-A mRNA 5′-UTRs and regulates its translation, which increases cell proliferation and contributes to tumorigenesis in colorectal cancer.",
author = "Lai, {Chien Hsien} and Huang, {Yu Chuan} and Lee, {Jenq Chang} and Tseng, {Joseph Ta Chien} and Chang, {Kung Chao} and Chen, {Yen Ju} and Ding, {Nai Jhu} and Huang, {Pao Hsuan} and Chang, {Wen Chang} and Lin, {Bo Wen} and Chen, {Ruo Yu} and Wang, {Yu Chu} and Lai, {Yi Chien} and Hung, {Liang Yi}",
note = "Funding Information: The authors thank Nature Publishing Group language editing for help with English editing. The authors also thank Insight Genomics (Tainan, Taiwan) in conducting Ribosomal profiling experiment and data analysis. This work was supported by grant MOST 103-2320-B-006-037, 104-2622-B-006-010-CC1, 104-2320-B-006-020-MY3 and 105-2320-B-006-051 from the Ministry of Science and Technology (Taipei, Taiwan). Funding Information: Acknowledgements. The authors thank Nature Publishing Group language editing for help with English editing. The authors also thank Insight Genomics (Tainan, Taiwan) in conducting Ribosomal profiling experiment and data analysis. This work was supported by grant MOST 103-2320-B-006-037, 104-2622-B-006-010-CC1, 104-2320-B-006-020-MY3 and 105-2320-B-006-051 from the Ministry of Science and Technology (Taipei, Taiwan). Publisher Copyright: {\textcopyright} 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.",
year = "2017",
doi = "10.1038/cddis.2016.479",
language = "English",
volume = "8",
journal = "Cell Death and Disease",
issn = "2041-4889",
publisher = "Nature Publishing Group",
number = "1",
}