Transplanted bone marrow-derived circulating PDGFRα+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft

Shin Iinuma, Eriko Aikawa, Katsuto Tamai, Ryo Fujita, Yasushi Kikuchi, Takenao Chino, Junichi Kikuta, John A. McGrath, Jouni Uitto, Masaru Ishii, Hajime Iizuka, Yasufumi Kaneda

研究成果: Article同行評審

62 引文 斯高帕斯(Scopus)

摘要

Recessive dystrophic epidermolysis bullosa (RDEB) is an intractable genetic blistering skin disease in which the epithelial structure easily separates from the underlying dermis because of genetic loss of functional type VII collagen (Co17) in the cutaneous basement membrane zone. Recent studies have demonstrated that allogeneic bone marrow transplantation (BMT) ameliorates the skin blistering phenotype of RDEB patients by restoring Co17. However, the exact therapeutic mechanism of BMT in RDEB remains unclear. In this study, we investigated the roles of transplanted bone marrow-derived circulating mesenchymal cells in RDEB (Co17-null) mice. In wild-type mice with prior GFP-BMT after lethal irradiation, lineage-negative/GFP-positive (Lin-/GFP+) cells, including platelet-derived growth factor receptor α-positive (PDGFRα+) mesenchymal cells, specifically migrated to skin grafts from RDEB mice and expressed Co17. Vascular endothelial cells and follicular keratinocytes in the deep dermis of the skin grafts expressed SDF-1α, and the bone marrow-derived PDGFRα+ cells expressed CXCR4 on their surface. Systemic administration of the CXCR4 antagonist AMD3100 markedly decreased the migration of bone marrow-derived PDGFRα+ cells into the skin graft, resulting in persistent epidermal detachment with massive necrosis and inflammation in the skin graft of RDEB mice; without AMD3100 administration, Co17 was significantly supplemented to ameliorate the pathogenic blistering phenotype. Collectively, these data suggest that the SDF1α/CXCR4 signaling axis induces transplanted bone marrow-derived circulating PDGFRα+ mesenchymal cells to migrate and supply functional Co17 to regenerate RDEB skin.

原文English
頁(從 - 到)1996-2003
頁數8
期刊Journal of Immunology
194
發行號4
DOIs
出版狀態Published - 2015 2月 15

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 免疫學

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