Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu; Ming Yang Lin; Kang Yi Lien; Lein Yu Hung; Fong Yu Cheng; Chih Chia Huang; Chen Sheng Yeh; Huan Yao Lei; Gwo Bin Lee

doi:10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu, Ming Yang Lin, Kang Yi Lien, Lein Yu Hung, Fong Yu Cheng, Chih Chia Huang, Chen Sheng Yeh, Huan Yao Lei, Gwo Bin Lee

研究成果: Conference contribution

摘要

This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl₂ solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

原文	English
主出版物標題	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
頁面	117-120
頁數	4
DOIs	https://doi.org/10.1109/NEMS.2011.6017309
出版狀態	Published - 2011
事件	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan 持續時間: 2011 2月 20 → 2011 2月 23

出版系列

名字	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
國家/地區	Taiwan
城市	Kaohsiung
期間	11-02-20 → 11-02-23

All Science Journal Classification (ASJC) codes

原子與分子物理與光學

UN SDG

此研究成果有助於以下永續發展目標

存取文件

10.1109/NEMS.2011.6017309

其它文件與鏈接

引用此

Hsu, R. C., Lin, M. Y., Lien, K. Y., Hung, L. Y., Cheng, F. Y., Huang, C. C., Yeh, C. S., Lei, H. Y., & Lee, G. B. (2011). Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. 於 NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (頁 117-120). 文章 6017309 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017309

Hsu, Ruo Chi ; Lin, Ming Yang ; Lien, Kang Yi 等. / Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. 頁 117-120 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

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title = "Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis",

abstract = "This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.",

author = "Hsu, {Ruo Chi} and Lin, {Ming Yang} and Lien, {Kang Yi} and Hung, {Lein Yu} and Cheng, {Fong Yu} and Huang, {Chih Chia} and Yeh, {Chen Sheng} and Lei, {Huan Yao} and Lee, {Gwo Bin}",

year = "2011",

doi = "10.1109/NEMS.2011.6017309",

language = "English",

isbn = "9781612847757",

series = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

pages = "117--120",

booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

}

Hsu, RC, Lin, MY, Lien, KY, Hung, LY, Cheng, FY, Huang, CC , Yeh, CS, Lei, HY & Lee, GB 2011, Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. 於 NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017309, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, 頁 117-120, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. / Hsu, Ruo Chi; Lin, Ming Yang; Lien, Kang Yi 等.
NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 117-120 6017309 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

研究成果: Conference contribution

TY - GEN

T1 - Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

AU - Hsu, Ruo Chi

AU - Lin, Ming Yang

AU - Lien, Kang Yi

AU - Hung, Lein Yu

AU - Cheng, Fong Yu

AU - Huang, Chih Chia

AU - Yeh, Chen Sheng

AU - Lei, Huan Yao

AU - Lee, Gwo Bin

PY - 2011

Y1 - 2011

N2 - This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

AB - This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

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M3 - Conference contribution

AN - SCOPUS:80053338594

SN - 9781612847757

T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

SP - 117

EP - 120

BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011

Y2 - 20 February 2011 through 23 February 2011

ER -

Hsu RC, Lin MY, Lien KY, Hung LY, Cheng FY, Huang CC 等. Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. 於 NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 117-120. 6017309. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). doi: 10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

摘要

出版系列

Other

All Science Journal Classification (ASJC) codes

UN SDG

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