Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu, Ming Yang Lin, Kang Yi Lien, Lein Yu Hung, Fong Yu Cheng, Chih Chia Huang, Chen Sheng Yeh, Huan Yao Lei, Gwo Bin Lee

研究成果: Conference contribution

摘要

This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

原文English
主出版物標題NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
頁面117-120
頁數4
DOIs
出版狀態Published - 2011
事件6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
持續時間: 2011 二月 202011 二月 23

出版系列

名字NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
國家/地區Taiwan
城市Kaohsiung
期間11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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