Turbidimetric microassay for macrophage-mediated antibody-dependent cellular cytotoxicity

John A. Rummage, Nan Shan Chang, Richard W. Leu

研究成果: Article

13 引文 斯高帕斯(Scopus)

摘要

An improved microassay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (ADCC) has been developed. The method is based on the turbidimetric measurement of sheep erythrocyte or nucleated (L1210) target cell suspensions at 630 nm with an automatic microtiter plate densitometer. The novel method was applied to demonstrate dose-related increases in murine macrophage mediated ADCC with varying antibody concentration, effector:target ratio, and incubation time. Advantages of the turbidimetric method were shown over the 51Cr-labeled target cell method by direct comparisons in that the new method was 2-4 times more sensitive and allowed repeated readings of the same plate after various incubation time intervals. The method provides further advantages of (1) elimination of the need for expensive and hazardous radioactive materials, (2) relative ease and rapidity in which experiments may be performed and quantitated, (3) sensitivity and reproducibility, and (4) versatility of the assay for measuring cytotoxicity of either erythrocyte or nucleated target cells.

原文English
頁(從 - 到)39-44
頁數6
期刊Journal of Immunological Methods
86
發行號1
DOIs
出版狀態Published - 1986 一月 22

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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