Turn on the Mtr pathway genes under placi promoter in shewanella oneidensis MR-1

I. Son Ng, Yanlan Guo, Yunli Zhou, Jhe Wei Wu, Shih I. Tan, Ying Chen Yi

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Background: Shewanella genus is famous for applications like electron transfer in microbe fuel cells and bioremediation of heavy metals through the Mtr pathway. A potential way to enhance the electron genesis ability of Shewanella is to express exogenous mtr genes via recombinant DNA technology. Thus, to design and develop expression vectors capable of replicating in Shewanella and enhance the genetic toolbox of the same is important. Result: In this study, a plasmid construct with a replication origin, repB, and pLacI promoter is reported for the first time to drive the expression of green fluorescent protein in S. oneidensis MR-1. Based on the same vector, the Mtr pathway genes mtrA, mtrC, and mtrCAB were also successfully expressed in MR-1. The recombinant strains had higher ferric reductase activity compared to the wild type. The highest enzymatic activity of 508.33 U/L in genetic Shewanella with mtrC gene is obtained, which is 1.53-fold higher than that of wild strain. The plasmids were stable up to 90 generations. Conclusion: We have demonstrated an expression system based on pLacI promoter and repB ori in Shewanella. Consequently, the combination of repB and pLacI will have great potential in Shewanella to turn on expression of different genes constitutively.

原文English
文章編號35
期刊Bioresources and Bioprocessing
5
發行號1
DOIs
出版狀態Published - 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Biomedical Engineering
  • Renewable Energy, Sustainability and the Environment

指紋 深入研究「Turn on the Mtr pathway genes under placi promoter in shewanella oneidensis MR-1」主題。共同形成了獨特的指紋。

引用此