Tyrosinase inhibitory activity of supernatant and semi-purified extracts from squid pen fermented with Burkholderia cepacia TKU025

Tzu Wen Liang, Yun Chen Lee, San Lang Wang

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Reports of tyrosinase inhibitors from microorganisms are rare. The tyrosinase inhibitory activity was found from the culture supernatant of Burkholderia cepacia TKU025 with 1 % squid pen as the sole carbon/nitrogen source. The fourth-day supernatant showed the strongest tyrosinase inhibitory activity (500 U/ml) and the highest total phenolic content (789 μg/ml GAE). The supernatant was further purified by liquid-liquid partition, and it was found that the methanol extract exhibited the lowest IC50 value of 2 μg/ml in the tyrosinase inhibitory activity assay and was compared with the positive control kojic acid and vitamin C. Significant associations between the tyrosinase inhibitory potency and the total phenolic content were found for the supernatant, extract, and fractions. The tyrosinase inhibitory activity persisted even when it was treated with acidic or alkaline conditions of pH 2 or 12. The tyrosinase inhibitory activity remained at 100 %, even after treatment at 100°C for 60 min. The tyrosinase inhibitor also showed antimicrobial activity against Escherichia coli, Bacillus cereus, Aspergillus fumigatus, and Fusarium oxysporum.

原文English
文章編號1725
頁(從 - 到)6105-6116
頁數12
期刊Research on Chemical Intermediates
41
發行號9
DOIs
出版狀態Published - 2015 九月 6

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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