Ultrasound therapy reduces persistent post-thoracotomy tactile allodynia and spinal substance P expression in rats

Heng Teng Lin, Chong Chi Chiu, Chen Chih Liu, Yu Wen Chen, Jhi Joung Wang, Ching-Hsia Hung

研究成果: Article

摘要

Background Therapeutic ultrasound (TU) alleviates nerve injury-associated pain, while the molecular mechanisms are less clear. This is an investigator-initiated experimental study to evaluate the mechanisms and effects of ultrasound on prolonged post-thoracotomy pain in a rodent model. Methods The rats were randomly separated into four groups (n=8 per group): sham-operation (sham; group 1), thoracotomy and rib retraction (TRR; group 2), and TRR procedure followed by TU (TRR+TU-3; group 3) or TU with the ultrasound power turned off (TRR+TU-0; group 4). TU was delivered daily, beginning on postoperative day 11 (POD 11) for the next 2 weeks. Mechanical sensitivity, subcutaneous tissue temperature, and spinal substance P and interleukin-1 beta (IL-1β) were evaluated on PODs 11 and 23. Results Group 3, which received ultrasound treatment (3 MHz; 1.0 W/cm 2) for 5 min each day, demonstrated higher mechanical withdrawal thresholds when compared with the group without ultrasound intervention (group 2) or sham ultrasound (group 4). Ultrasound treatment also inhibited the upregulation of spinal substance P and IL-1β measured from spinal cord dorsal horns extract and increased subcutaneous temperature. Conclusions The results of this study suggest an increase in mechanical withdrawal thresholds and subcutaneous temperature, as well as a downregulation of spinal substance P and IL-1β, in the group which received ultrasound treatment. The regulation of spinal substance P and IL-1β may mediate potential effects of this non-invasive treatment.

原文English
頁(從 - 到)604-608
頁數5
期刊Regional anesthesia and pain medicine
44
發行號5
DOIs
出版狀態Published - 2019 五月 1

指紋

Hyperalgesia
Thoracotomy
Substance P
Interleukin-1beta
Therapeutics
Temperature
Pain
Subcutaneous Tissue
Ribs
Rodentia
Up-Regulation
Down-Regulation
Research Personnel

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

引用此文

Lin, Heng Teng ; Chiu, Chong Chi ; Liu, Chen Chih ; Chen, Yu Wen ; Wang, Jhi Joung ; Hung, Ching-Hsia. / Ultrasound therapy reduces persistent post-thoracotomy tactile allodynia and spinal substance P expression in rats. 於: Regional anesthesia and pain medicine. 2019 ; 卷 44, 編號 5. 頁 604-608.
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abstract = "Background Therapeutic ultrasound (TU) alleviates nerve injury-associated pain, while the molecular mechanisms are less clear. This is an investigator-initiated experimental study to evaluate the mechanisms and effects of ultrasound on prolonged post-thoracotomy pain in a rodent model. Methods The rats were randomly separated into four groups (n=8 per group): sham-operation (sham; group 1), thoracotomy and rib retraction (TRR; group 2), and TRR procedure followed by TU (TRR+TU-3; group 3) or TU with the ultrasound power turned off (TRR+TU-0; group 4). TU was delivered daily, beginning on postoperative day 11 (POD 11) for the next 2 weeks. Mechanical sensitivity, subcutaneous tissue temperature, and spinal substance P and interleukin-1 beta (IL-1β) were evaluated on PODs 11 and 23. Results Group 3, which received ultrasound treatment (3 MHz; 1.0 W/cm 2) for 5 min each day, demonstrated higher mechanical withdrawal thresholds when compared with the group without ultrasound intervention (group 2) or sham ultrasound (group 4). Ultrasound treatment also inhibited the upregulation of spinal substance P and IL-1β measured from spinal cord dorsal horns extract and increased subcutaneous temperature. Conclusions The results of this study suggest an increase in mechanical withdrawal thresholds and subcutaneous temperature, as well as a downregulation of spinal substance P and IL-1β, in the group which received ultrasound treatment. The regulation of spinal substance P and IL-1β may mediate potential effects of this non-invasive treatment.",
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Ultrasound therapy reduces persistent post-thoracotomy tactile allodynia and spinal substance P expression in rats. / Lin, Heng Teng; Chiu, Chong Chi; Liu, Chen Chih; Chen, Yu Wen; Wang, Jhi Joung; Hung, Ching-Hsia.

於: Regional anesthesia and pain medicine, 卷 44, 編號 5, 01.05.2019, p. 604-608.

研究成果: Article

TY - JOUR

T1 - Ultrasound therapy reduces persistent post-thoracotomy tactile allodynia and spinal substance P expression in rats

AU - Lin, Heng Teng

AU - Chiu, Chong Chi

AU - Liu, Chen Chih

AU - Chen, Yu Wen

AU - Wang, Jhi Joung

AU - Hung, Ching-Hsia

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Background Therapeutic ultrasound (TU) alleviates nerve injury-associated pain, while the molecular mechanisms are less clear. This is an investigator-initiated experimental study to evaluate the mechanisms and effects of ultrasound on prolonged post-thoracotomy pain in a rodent model. Methods The rats were randomly separated into four groups (n=8 per group): sham-operation (sham; group 1), thoracotomy and rib retraction (TRR; group 2), and TRR procedure followed by TU (TRR+TU-3; group 3) or TU with the ultrasound power turned off (TRR+TU-0; group 4). TU was delivered daily, beginning on postoperative day 11 (POD 11) for the next 2 weeks. Mechanical sensitivity, subcutaneous tissue temperature, and spinal substance P and interleukin-1 beta (IL-1β) were evaluated on PODs 11 and 23. Results Group 3, which received ultrasound treatment (3 MHz; 1.0 W/cm 2) for 5 min each day, demonstrated higher mechanical withdrawal thresholds when compared with the group without ultrasound intervention (group 2) or sham ultrasound (group 4). Ultrasound treatment also inhibited the upregulation of spinal substance P and IL-1β measured from spinal cord dorsal horns extract and increased subcutaneous temperature. Conclusions The results of this study suggest an increase in mechanical withdrawal thresholds and subcutaneous temperature, as well as a downregulation of spinal substance P and IL-1β, in the group which received ultrasound treatment. The regulation of spinal substance P and IL-1β may mediate potential effects of this non-invasive treatment.

AB - Background Therapeutic ultrasound (TU) alleviates nerve injury-associated pain, while the molecular mechanisms are less clear. This is an investigator-initiated experimental study to evaluate the mechanisms and effects of ultrasound on prolonged post-thoracotomy pain in a rodent model. Methods The rats were randomly separated into four groups (n=8 per group): sham-operation (sham; group 1), thoracotomy and rib retraction (TRR; group 2), and TRR procedure followed by TU (TRR+TU-3; group 3) or TU with the ultrasound power turned off (TRR+TU-0; group 4). TU was delivered daily, beginning on postoperative day 11 (POD 11) for the next 2 weeks. Mechanical sensitivity, subcutaneous tissue temperature, and spinal substance P and interleukin-1 beta (IL-1β) were evaluated on PODs 11 and 23. Results Group 3, which received ultrasound treatment (3 MHz; 1.0 W/cm 2) for 5 min each day, demonstrated higher mechanical withdrawal thresholds when compared with the group without ultrasound intervention (group 2) or sham ultrasound (group 4). Ultrasound treatment also inhibited the upregulation of spinal substance P and IL-1β measured from spinal cord dorsal horns extract and increased subcutaneous temperature. Conclusions The results of this study suggest an increase in mechanical withdrawal thresholds and subcutaneous temperature, as well as a downregulation of spinal substance P and IL-1β, in the group which received ultrasound treatment. The regulation of spinal substance P and IL-1β may mediate potential effects of this non-invasive treatment.

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