Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus

Shih Yao Chen, Ming Fei Liu, Pin Yu Kuo, Chrong Reen Wang

研究成果: Article

3 引文 (Scopus)

摘要

Elevated IL-17 levels with higher Th17 numbers are identified in systemic lupus erythematosus (SLE). STAT3 signaling plays a crucial role in the Th17 generation, and SOCS3 negatively regulates their formation. We investigated IL-17, STAT3, and SOCS3 expression, and analyzed their correlations to elucidate the regulatory mechanisms of IL-17 production in SLE. This study enrolled 32 patients, and venous mononuclear cells (MNCs) were isolated with further purification of CD4-positive T cells. IL-17 and SOCS3 levels were measured by real-time quantitative PCR, and pSTAT3/STAT3 expression was analyzed by immunoblot. Elevated IL-17 and SOCS3 levels were identified in lupus patients. There were higher IL-17 levels in lupus nephritis (class IV) than in SLE without renal involvement. Positive correlations were found between IL-17 levels and SOCS3 expression, lupus activity (SLEDAI-2K), or daily proteinuria. There were higher intensities of pSTAT3/β-actin and STAT3/β-actin in SLE, and a positive correlation between IL-17 expression and pSTAT3/β-actin or STAT3/β-actin intensity. Lupus nephritis (class IV) had higher STAT3/β-actin intensity than SLE without renal involvement. These results suggest upregulated STAT3/IL-17 expression in lupus patients. Such findings might facilitate the development of novel compounds and the application of existing therapeutics targeting the STAT3/IL-17 signal in SLE.

原文English
頁(從 - 到)1361-1366
頁數6
期刊Clinical Rheumatology
38
發行號5
DOIs
出版狀態Published - 2019 五月 1

指紋

Interleukin-17
Systemic Lupus Erythematosus
Actins
Lupus Nephritis
Kidney
Proteinuria
Real-Time Polymerase Chain Reaction
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Rheumatology

引用此文

Chen, Shih Yao ; Liu, Ming Fei ; Kuo, Pin Yu ; Wang, Chrong Reen. / Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus. 於: Clinical Rheumatology. 2019 ; 卷 38, 編號 5. 頁 1361-1366.
@article{430fe9efbcc4487cbdd900f76e87b821,
title = "Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus",
abstract = "Elevated IL-17 levels with higher Th17 numbers are identified in systemic lupus erythematosus (SLE). STAT3 signaling plays a crucial role in the Th17 generation, and SOCS3 negatively regulates their formation. We investigated IL-17, STAT3, and SOCS3 expression, and analyzed their correlations to elucidate the regulatory mechanisms of IL-17 production in SLE. This study enrolled 32 patients, and venous mononuclear cells (MNCs) were isolated with further purification of CD4-positive T cells. IL-17 and SOCS3 levels were measured by real-time quantitative PCR, and pSTAT3/STAT3 expression was analyzed by immunoblot. Elevated IL-17 and SOCS3 levels were identified in lupus patients. There were higher IL-17 levels in lupus nephritis (class IV) than in SLE without renal involvement. Positive correlations were found between IL-17 levels and SOCS3 expression, lupus activity (SLEDAI-2K), or daily proteinuria. There were higher intensities of pSTAT3/β-actin and STAT3/β-actin in SLE, and a positive correlation between IL-17 expression and pSTAT3/β-actin or STAT3/β-actin intensity. Lupus nephritis (class IV) had higher STAT3/β-actin intensity than SLE without renal involvement. These results suggest upregulated STAT3/IL-17 expression in lupus patients. Such findings might facilitate the development of novel compounds and the application of existing therapeutics targeting the STAT3/IL-17 signal in SLE.",
author = "Chen, {Shih Yao} and Liu, {Ming Fei} and Kuo, {Pin Yu} and Wang, {Chrong Reen}",
year = "2019",
month = "5",
day = "1",
doi = "10.1007/s10067-019-04467-8",
language = "English",
volume = "38",
pages = "1361--1366",
journal = "Clinical Rheumatology",
issn = "0770-3198",
publisher = "Springer London",
number = "5",

}

Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus. / Chen, Shih Yao; Liu, Ming Fei; Kuo, Pin Yu; Wang, Chrong Reen.

於: Clinical Rheumatology, 卷 38, 編號 5, 01.05.2019, p. 1361-1366.

研究成果: Article

TY - JOUR

T1 - Upregulated expression of STAT3/IL-17 in patients with systemic lupus erythematosus

AU - Chen, Shih Yao

AU - Liu, Ming Fei

AU - Kuo, Pin Yu

AU - Wang, Chrong Reen

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Elevated IL-17 levels with higher Th17 numbers are identified in systemic lupus erythematosus (SLE). STAT3 signaling plays a crucial role in the Th17 generation, and SOCS3 negatively regulates their formation. We investigated IL-17, STAT3, and SOCS3 expression, and analyzed their correlations to elucidate the regulatory mechanisms of IL-17 production in SLE. This study enrolled 32 patients, and venous mononuclear cells (MNCs) were isolated with further purification of CD4-positive T cells. IL-17 and SOCS3 levels were measured by real-time quantitative PCR, and pSTAT3/STAT3 expression was analyzed by immunoblot. Elevated IL-17 and SOCS3 levels were identified in lupus patients. There were higher IL-17 levels in lupus nephritis (class IV) than in SLE without renal involvement. Positive correlations were found between IL-17 levels and SOCS3 expression, lupus activity (SLEDAI-2K), or daily proteinuria. There were higher intensities of pSTAT3/β-actin and STAT3/β-actin in SLE, and a positive correlation between IL-17 expression and pSTAT3/β-actin or STAT3/β-actin intensity. Lupus nephritis (class IV) had higher STAT3/β-actin intensity than SLE without renal involvement. These results suggest upregulated STAT3/IL-17 expression in lupus patients. Such findings might facilitate the development of novel compounds and the application of existing therapeutics targeting the STAT3/IL-17 signal in SLE.

AB - Elevated IL-17 levels with higher Th17 numbers are identified in systemic lupus erythematosus (SLE). STAT3 signaling plays a crucial role in the Th17 generation, and SOCS3 negatively regulates their formation. We investigated IL-17, STAT3, and SOCS3 expression, and analyzed their correlations to elucidate the regulatory mechanisms of IL-17 production in SLE. This study enrolled 32 patients, and venous mononuclear cells (MNCs) were isolated with further purification of CD4-positive T cells. IL-17 and SOCS3 levels were measured by real-time quantitative PCR, and pSTAT3/STAT3 expression was analyzed by immunoblot. Elevated IL-17 and SOCS3 levels were identified in lupus patients. There were higher IL-17 levels in lupus nephritis (class IV) than in SLE without renal involvement. Positive correlations were found between IL-17 levels and SOCS3 expression, lupus activity (SLEDAI-2K), or daily proteinuria. There were higher intensities of pSTAT3/β-actin and STAT3/β-actin in SLE, and a positive correlation between IL-17 expression and pSTAT3/β-actin or STAT3/β-actin intensity. Lupus nephritis (class IV) had higher STAT3/β-actin intensity than SLE without renal involvement. These results suggest upregulated STAT3/IL-17 expression in lupus patients. Such findings might facilitate the development of novel compounds and the application of existing therapeutics targeting the STAT3/IL-17 signal in SLE.

UR - http://www.scopus.com/inward/record.url?scp=85061608402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061608402&partnerID=8YFLogxK

U2 - 10.1007/s10067-019-04467-8

DO - 10.1007/s10067-019-04467-8

M3 - Article

C2 - 30767092

AN - SCOPUS:85061608402

VL - 38

SP - 1361

EP - 1366

JO - Clinical Rheumatology

JF - Clinical Rheumatology

SN - 0770-3198

IS - 5

ER -