Using an electro-microchip, a nanogold probe, and silver enhancement in an immunoassay

Chia Hsien Yeh, Hao Hsuan Huang, Tsung Chain Chang, Hong Ping Lin, Yu Cheng Lin

研究成果: Article同行評審

31 引文 斯高帕斯(Scopus)

摘要

This paper presents a novel immunoassay that uses an electro-microchip to detect the immuno-reaction signal, gold nanoparticles (ANPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. This study is based on the direct immunoassay (two-layer format) and the sandwich immunoassay (three-layer format). The ANPs were introduced into the electro-microchip by the specific binding of the antibodies-ANPs conjugates and then were coupled with silver enhancement to produce black spots of silver metal. The silver precipitation constructs a "bridge" between two electrodes of the electro-microchip allowing electrons to pass. The variation of impedance can be easily measured with a commercial LCR meter. Various gap sizes (20, 50, 100, and 200 μm) of the electrodes of electro-microchips were designed for the sensitivity study. The experimental data show that a chip with a 20 μm gap has the highest sensitivity. There was a significant difference in impedance between the experiment sample and the negative control after 10 min of reaction time. The proposed method requires less time and fewer steps than the conventional enzyme-linked immunosorbent assay (ELISA). In addition, it shows a high detection sensitivity (10 μg/mL of 1st antibody (IgG) immobilized on slides and 1 ng/mL of antigen (protein A)). There is a clear distinction between the signal intensity and the logarithm of the sample concentration. The proposed new immunoassay method has potential applications in proteomics research and clinical diagnosis. Crown

原文English
頁(從 - 到)1661-1666
頁數6
期刊Biosensors and Bioelectronics
24
發行號6
DOIs
出版狀態Published - 2009 二月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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