TY - GEN
T1 - Using magnetic nanoparticles to enhance gene transfection on magneto-electroporation microchips
AU - Li, Min
AU - Lin, Yu Cheng
AU - Su, Kai Chun
PY - 2006
Y1 - 2006
N2 - This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05 %) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.
AB - This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05 %) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.
UR - https://www.scopus.com/pages/publications/34548318340
UR - https://www.scopus.com/pages/publications/34548318340#tab=citedBy
U2 - 10.4028/0-87849-990-3.661
DO - 10.4028/0-87849-990-3.661
M3 - Conference contribution
AN - SCOPUS:34548318340
SN - 0878499903
SN - 9780878499908
T3 - Materials Science Forum
SP - 661
EP - 666
BT - Progress on Advanced Manufacture for Micro/Nano Technology 2005 - Proceedings of the 2005 International Conference on Advanced Manufacture
PB - Trans Tech Publications Ltd
T2 - 2005 International Conference on Advanced Manufacture, ICAM2005
Y2 - 28 November 2005 through 2 December 2005
ER -