Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips

Ming Kai Liu, Hung Yi Chen, Yi Lung Wang, Shen Shing Tsai, Min Li, Yu Cheng Lin

研究成果: Conference contribution

摘要

This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05%) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.

原文English
主出版物標題Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
頁面3023-3026
頁數4
DOIs
出版狀態Published - 2007
事件33rd Annual Conference of the IEEE Industrial Electronics Society, IECON - Taipei, Taiwan
持續時間: 2007 11月 52007 11月 8

出版系列

名字IECON Proceedings (Industrial Electronics Conference)

Other

Other33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
國家/地區Taiwan
城市Taipei
期間07-11-0507-11-08

All Science Journal Classification (ASJC) codes

  • 控制與系統工程
  • 電氣與電子工程

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