Using micro-patterned sensors and cell self-assembly for measuring the oxygen consumption rate of single cells

James R. Etzkorn, Wen Chung Wu, Zhiyuan Tian, Prince Kim, Sei Hum Jang, Deirdre R. Meldrum, Alex K.Y. Jen, Babak A. Parviz

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

We present a method for self-assembling arrays of live single cells on a glass chip using a photopatternable polymer to form micro-traps. We have studied the single-cell self-assembly method and optimized the process to obtain a 52% yield of single-trapped cells. We also report a method to measure the oxygen consumption rate of a single cell using micro-patterned sensors. These molecular oxygen sensors were fabricated around each micro-trap allowing optical interrogation of oxygen concentration in the immediate environment of the trapped cell. Micromachined micro-wells were then used to seal the trap, sensor and cell in order to determine the oxygen consumption rate of single cells. These techniques reported here add to the collection of tools for performing 'singe-cell' biology. An oxygen consumption rate of 1.05 ± 0.28 fmol min-1 was found for a data set consisting of 25 single A549 cells.

原文English
文章編號095017
期刊Journal of Micromechanics and Microengineering
20
發行號9
DOIs
出版狀態Published - 2010 9月

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 材料力學
  • 機械工業
  • 電氣與電子工程

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