Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection

Chia Hsien Yeh, Kuan Feng Su, Yu Cheng Lin, Pi Lan Shen

研究成果: Conference contribution

摘要

In this study, a biosensor was successfully used to detect the impedance signals of various carcinoembryonic antigen (CEA) concentrations based on the developed sandwich-immunoassay method. CEA is a type of protein molecule that can be found in many different cells of the body, but is typically associated with certain tumors; therefore, CEA concentration detection is important for quantitative analysis. In this study, the gold nanoparticles (AuNPs) were introduced into a biosensor through the specific binding of antibodies, thus creating a bridge between two electrodes allowing electrons to move between them. When the concentration of the anti-CEA antibody-colloidal gold conjugates was diluted 30 times, the detectable limit for CEA concentration was 1 ng/mL with 1 μg/mL of anti-CEA antibody. Therefore, the developed biosensor is advantageous because it is effective with small sample volumes (25 μL), is a form of rapid quantitative measurement, and works with detection concentrations range in clinical diagnosis.

原文English
主出版物標題IEEE SENSORS 2013 - Proceedings
發行者IEEE Computer Society
ISBN(列印)9781467346405
DOIs
出版狀態Published - 2013 一月 1
事件12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
持續時間: 2013 十一月 42013 十一月 6

出版系列

名字Proceedings of IEEE Sensors

Other

Other12th IEEE SENSORS 2013 Conference
國家/地區United States
城市Baltimore, MD
期間13-11-0413-11-06

All Science Journal Classification (ASJC) codes

  • 電氣與電子工程

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